pFGC-pcoCas9
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质粒名称:pFGC-pcoCas9 质粒编号:52256 质粒备注: A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA 载体名称:pFGC-RCS 载体类型:CRISPR ; Plant expression 筛选标记:Basta 原核抗性:卡那霉素,50μg/mL 培养温度:37℃ 克隆菌株:DH5alpha 高拷贝/低拷贝:未知 插入基因:35SPPDK:pcoCas9:NOS cassette 基因别名:Cas9 基因别名:plant codon optimized Cas9 物种来源:Synthetic 启动子:constitutive 35SPPDK promoter 克隆方法:限制性内切酶 5'克隆位点:StuI (not destroyed) 3'克隆位点:StuI (not destroyed) 5'测序引物:Sequencing primer for multiple cloning sites (from somewhere close to the EcoRI site): 5’ AATAAAAACTGACTCGGA 3’ 3'测序引物:Sequencing primer for 35SPPDK:pcoCas9:NOS (from somewhere close to the 35SPPDK promoter): 5’ AGTTGGGTAACGCCAGGG 3’ 质粒图谱: 用户评论 产品评分 目前评分共0人 产品质量
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