A-427人肺癌细胞(2021年新引进)
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目录号 Serial | SCSP-579 | 标识符 Identifier | CSTR:19375.09.3101HUMSCSP579 | 描述 Description | 52岁白人男性的肺癌细胞,在裸鼠中具有致瘤性。 质量控制: 支原体检测结果:本批次已通过本库支原体检测,结果为阴性; STR检测结果: Amelogenin: X,Y CSF1PO: 10,12 D13S317: 11,12 D16S539: 11,13 D5S818: 12,12 D7S820: 8,12 THO1: 9,9 TPOX: 8,11 vWA: 17,17 | 动物种别 Organism | 人,白人 | 性别 Gender | 男性,52岁 | 组织来源 Tissue and Cell Type | 肺 | 形态 Morphology | 贴壁 | 培养基和添加剂 Complete Growth Medium and Culture Conditions | 人肺癌细胞 A-427 完全培养液 配方(100 ml): MEM (Invitrogen, 11090081) 87 ml FBS (Gibco)10 ml Glutamax (Invitrogen 35050061) 1 ml Non-essential Amino Acids, 100 (Invitrogen, 11140050) 1 ml Sodium Pyruvate 100 mM Solution (Invitrogen, 11360070) 1 ml 我库冻存时,每支冻存管约含7105 细胞量,体积为500 μl,预期存活率70%,建议复苏至1个T25培养瓶中。 参考传代比例:1:3 参考传代周期:4-6天 参考换液频率:每2-3天换1次液 冻存液配方:完全培养液95%,DMSO 5% 气相:空气,95%;二氧化碳,5%。温度:37摄氏度 | 供应限制 Permits and Restrictions | A。仅供研究之用。 | REFERENCE | Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977.PubMed:833871Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977.PubMed:77210034Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973.PubMed:4357758 |
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