Ned 19 是一种选择性膜透性非竞争性NAADP拮抗剂，抑制 NAADP 介导的Ca2+信号传导，IC50为 65 nM。 Ned 19 强烈抑制小鼠的肿瘤生长和血管形成以及肺转移。
| 生物活性 | Ned 19 is a selective membrane-permeant non competitiveNAADPantagonist and inhibits NAADP-mediatedCa2+signaling, with anIC50of 65 nM[1]. Ned 19 strongly inhibits tumor growth and vascularization as well as lung metastases in mice[2]. |
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体外研究
(In Vitro) | Ned 19 (25-100 μM; 24-72 hours) reduces cell proliferation[2].
Ned 19 (25-100 μM; 24-72 hours) reduces markedly the cell number[2].
Ned 19 (25-100 μM; 24-72 hours) reduces the S phase percentage and increases of the G0/G1 phase percentage evaluated by cell cycle analysis[2].
Ned 19 (25-100 μM; 24-72 hours) induces cell apoptosis a time-dependent manner[2].
Ned 19 (25-100 μM; 24-72 hours) reduces expression of N-cadherin and increases expression of E-cadherin, affecting the cell migratory behavior[2].
Cell Proliferation Assay[2] | Cell Line: | B16 cells | | Concentration: | 25, 50, 100 μM | | Incubation Time: | 24, 48, 72 hours | | Result: | Reduced cell proliferation. |
Cell Viability Assay[2] | Cell Line: | B16 cells | | Concentration: | 25, 50, 100 μM | | Incubation Time: | 24, 48, 72 hours | | Result: | Reduced markedly the cell number. |
Cell Cycle Analysis[2] | Cell Line: | B16 cells | | Concentration: | 25, 50, 100 μM | | Incubation Time: | 24, 48, 72 hours | | Result: | Reduced the S phase percentage and increased of the G0/G1 phase percentage. |
Apoptosis Analysis[2] | Cell Line: | B16 cells | | Concentration: | 25, 50, 100 μM | | Incubation Time: | 24, 48, 72 hours | | Result: | Induced cell apoptosis a time-dependent manner. |
Western Blot Analysis[2] | Cell Line: | B16 cells | | Concentration: | 25, 50, 100 μM | | Incubation Time: | 24, 48, 72 hours | | Result: | Reduced expression of N-cadherin and increased expression of E-cadherin. |
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体内研究
(In Vivo) | Ned 19 (i.p.; 5 mg/Kg/every second day; 4 week) impaires severely tumor growth[2].
| Animal Model: | Adult male C57BL/6 mice[2] | | Dosage: | 5 mg/Kg | | Administration: | I.p.; every second day; 4 week | | Result: | Impaired severely tumor growth. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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