Sulforhodamine B sodium salt 是一种能够定量测定培养细胞的细胞蛋白量的荧光染料，。

Sulforhodamine B sodium salt is a fluorescent dye. It can be used from laser-induced fluorescence to the quantification of cellular proteins of cultured cells.

Sulforhodamine B (SRB) is often used as a membrane-impermeable polar tracer or used for cell density determination via the determination of cellular proteins (cytotoxicity assay). The SRB assay has been used to inexpensively conduct various screening assays to investigate cytotoxicity in cell-based studies. The protocol can be divided into four main steps: preparation of treatment, incubation of cells with treatment of choice, cell fixation, and SRB staining, and absorbance measurement. This assay is limited to manual or semiautomatic screening and can be used in an efficient and sensitive manner to test chemotherapeutic drugs or small molecules in adherent cells. It also has applications in evaluating the effects of gene expression modulation (knockdown, gene expression upregulation), as well as to study the effects of miRNA replacement on cell proliferation.

3520-42-1

C27H30N2NaO7S2

581.66

Acid Red 52;磺酰罗丹明B;Kiton Red 620

DMSO:50 mg/mL (86.11 mM),Need ultrasonic
H2O:20 mg/mL (34.44 mM),Need ultrasonic
Powder: -20°C for 3 years
In solvent: -80°C for 2 years