B-CD(生物素-环糊精;Bio-CD;Bio-β-CD；环糊精-生物素)

B-CD(mono-6-biotin-amino-6-deoxy-β-cyclodextrin）是一种生物素与β环糊精偶联的新型药物递送载体。生物素（BIO）被广泛用作一种很有前景的肿瘤靶向配体，其受体存在于许多癌细胞中，如肺癌、卵巢癌、结肠癌和乳腺癌等细胞，然而在正常细胞中几乎找不到；环糊精（CD）具有两个亲水的外表面，水溶性非常好，疏水内部通过超分子包合络合可以容纳各种难溶性药物或者化疗剂。B-CD具备生物素对癌细胞的选择性，同时也解决了难溶性药物的递送问题。

别名：单-6-生物素-氨基-6-脱氧-β-环糊精；生物素-单氨基-β-环糊精；β-环糊精-生物素；β-cyclodextrin-biotin；biotin-polyamine-β-CD；BCD;Bio-CD

存储：Store at -20°C

Background:

β-cyclodextrin-biotin的合成方法：通过环糊精分子引入氨基，再与生物素分子缩合。[1]

B-CD(生物素-环糊精)装载药物递送的可能方式：利用生物素对癌细胞的识别作用，把装载药物定向带入癌细胞，释放药物，定向杀死癌细胞[2]

应用案列分享：

Cytotoxicties (IC50) of PPT, PPT/β-CD inclusion complex and PPT/B-CD inclusion complex against MDA-M231 cell lines.

biotin-amino-6-deoxy-β-cyclodextrin inclusion complex. The possible  inclusion mode of podophyllotoxin/mono-6-biotin-amino-6-deoxy-  β-cyclodextrin complex was inferred from the Proton Nuclear Magnetic  Resonance and Rotating-frame Overhauser Effect Spectroscopy. Moreover, the cell uptake study showed that the introduction of biotin  increased the cellular uptake of rhodamine-B/mono-6-biotin-amino-6-  deoxy- β-cyclodextrin complex. Podophyllotoxin/mono-6-biotin-amino-6-deoxy- β-cyclodextrin demonstrated superior antitumor activity as compared with podophyllotoxin and podophyllotoxin/β-cyclodextrin in breast cancer. It may be concluded that the introduction of  biotin enhanced the selectivity of podophyllotoxin complex towards  tumor cells. Therefore, the biotin conjugated β-cyclodextrin based inclusion complex might be used as a potential anticancer drug carrier for  the treatment of cancer.[1]

In vitro cytotoxicity studies show that the cytotoxicity of luteolin was signifificantly enhanced after inclusion complexation with BCDs, which might be due to the increased cellular uptake of LU in cancer cell with better solubility. Moreover, they barely show cytotoxicity to normal cell, which is due to the enhanced tumor cell targeted ability by introduction of biotin. Thus, the biotin-polyamine-β-cyclodextrin might be a promising drug carrier, which is could be used in further tumor targeted clinical treatment.[2]

参考文献：

[1]Preparation, characterization and biological evaluation of β-cyclodextrin-biotin conjugate based podophyllotoxin complexEuropean Journal of Pharmaceutical Sciences 160 (2021) 105745

[2]A novel host-guest complex based on biotin functionalized polyamine-β-cyclodextrin for tumor targeted delivery of luteolin.Journal of Molecular Structure 1237 (2021) 130339