A more stable and less phototoxic form of (-)-blebbistatin
货号:ajcx28558
CAS:1621326-32-6
分子式:C18H15N3O4
分子量：337.3
溶解度:DMF: 20 mg/ml,DMF:PBS(pH 7.2)(1:1): 0.5 mg/ml,DMSO: 12.5 mg/ml
纯度：98%
存储：Store at -20°C
库存：现货

Background:

(S)-4'-nitro-Blebbistatin is a more stable and less phototoxic form of (-)-blebbistatin , which is a selective cell-permeable inhibitor of non-muscle myosin II ATPases.^1,2(-)-Blebbistatin rapidly and reversibly inhibits Mg-ATPase activity andin vitromotility of non-muscle myosin IIA and IIB for several species (IC₅₀s = 0.5-5 µM), while poorly inhibiting smooth muscle myosin (IC₅₀= 80 µM).³Through these effects, it blocks apoptosis-related bleb formation, directed cell migration, and cytokinesis in vertebrate cells. However, prolonged exposure to blue light (450-490 nm) results in degradation of blebbistatin to an inactive productviacytotoxic intermediates, which may be problematic for its use in fluorescent live cell imaging applications.^4,5The addition of a 4'-nitro group decreases the inherent fluorescence, stabilizes the molecule to circumvent its degradation by prolonged blue light exposure, and decreases its phototoxicity while retaining thein vitroandin vivoactivity of (-)-blebbistatin.^6,7(S)-4'-nitro-Blebbistatin has the same stereochemistry as the active (-)-blebbistatin enantiomer.

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