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Z-LEHD-FMK
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Z-LEHD-FMK图片
CAS NO:210345-04-3
规格:≥98%
包装与价格：
包装价格(元)
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
250mg电议

产品介绍
理化性质和储存条件
Molecular Weight (MW) 690.72
Formula C32H43FN6O10
CAS No. 210345-04-3 (free base);
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: ≥ 10mg/mL
Water: N/A
Ethanol: N/A
Chemical Name methyl (4S)-5-[[(2S)-1-[[(3S)-5-fluoro-1-methoxy-1,4-dioxopentan-3-yl]amino]-3-(1H-imidazol-5-yl)-1-oxopropan-2-yl]amino]-4-[[(2S)-4-methyl-2-(phenylmethoxycarbonylamino)pentanoyl]amino]-5-oxopentanoate
Synonyms Z-LEHD-FMK; Z-LEHD-FMK; Z LEHD-FMK; Z LEHD FMK; Z-LEHD FMK
SMILES Code CC(C)CC(C(=O)NC(CCC(=O)OC)C(=O)NC(CC1=CN=CN1)C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)OCC2=CC=CC=C2
实验参考方法
Target

Caspase-9

In VitroZ-LEHD-FMK (20 μM; pretreated for 30 min) completely protects HCT116 and 293 cells from TRAIL-induced toxicity[1]. Z-LEHD-FMK (20 μM ; 6 h) protects normal human hepatocytes from TRAIL-induced apoptosis[1]. Apoptosis Analysis[1] Cell Line: SW480, H460, HCT116 and 293 cells Concentration: 20 μM Incubation Time: Pretreated for 30 min Result: Protected HCT116 and 293 cells from TRAIL-induced apoptosis. Western Blot Analysis[1] Cell Line: HCT116, SW480 cells Concentration: 20 μM Incubation Time: 2 h Result: Protected procaspase 3 from cleavage in HCT116 cells but not in SW480 cells, especially at the 16-h time point.
In VivoZ-LEHD-FMK (0.8 μmol/kg; i.v. for 7 d) protects neurons, glia, myelin, axons, and intracellular organelles in spinal cord injury (SCI) rats[2]. Animal Model: Male Wistar albino rats (250-350 g) with SCI[2] Dosage: 0.8 μmol/kg Administration: I.v. for 1 or 7 days Result: Decreased the mean apoptotic cell count at 24 hours and 7 days postinjury.
References

[1]. The caspase 9 inhibitor Z-LEHD-FMK protects human liver cells while permitting death of cancer cells exposed to tumor necrosis factor-related apoptosis-inducing ligand. Cancer Res. 2000 Nov 15; 60(22): 6259-65.

[2]. Neuroprotection and functional recovery after application of the caspase-9 inhibitor z-LEHD-fmk in a rat model of traumatic spinal cord injury. J Neurosurg Spine. 2005 Mar; 2(3): 327-34.

[3]. Caspase inhibition and limitation of myocardial infarct size: protection against lethal reperfusion injury. Br J Pharmacol. 2000 May; 130(2): 197-200.