包装 | 价格(元) |
500μL | 电议 |
2 x 500μL | 电议 |
10 x 500μL | 电议 |
20 x 500μL | 电议 |
应用&特点 | 1.Safe and non-toxic: The unique oily macromolecule characteristics make it unable to penetrate the cell membrane and enter the cell. The Ames test result also shows that the mutagenicity of the dye is far less than EB.
2.High sensitivity: It is suitable for electrophoretic staining of fragments of various sizes and has little effect on nucleic acid migration. The sample has a strong fluorescence signal and a low background signal. 3.High stability: It is suitable for preparing agarose gel using microwave or other heating methods; it is extremely stable in acid or alkali buffer at room temperature and has strong light resistance. And not volatile! 4.Simple operation: it does not degrade during precast gel and electrophoresis, and can be directly observed with visible light gel transmission instrument. 5.Wide range of application: you can choose to stain before electrophoresis (gel staining) or after electrophoresis (bubble staining); apply to agarose gel or polyacrylamide gel electrophoresis; can be used for dsDNA, ssDNA or RNA staining. |
运输方式 | Ship with blue ice. |
储存条件 | Store at 2-8?C (Avoid direct sunlight), and is stable for up to 2 years. |
用途 | 仅供研究使用!不能用于人体。 |
文献引用 | |
产品描述 | Gsafe Red Plus Nucleic Acid Dye (10000X) is a red fluorescent nucleic acid stain that has been optimized and developed on the basis of Gelred with gel staining characteristics and is designed to replace the highly toxic staining agent ethidium bromide (EB) with higher clarity and sensitivity. Because Gsafe Red Plus has the same spectral characteristics as EB, it can be used to replace EB without changing any imaging system. If you use SYBR (such as SYBR Green I / SYBR Gold) stain and use a UV transilluminator to observe the gel, you can use Gelred Plus to replace the SYBR stain, without replacing the existing SYBR light. sheet. However, Plus cannot be sufficiently excited under a 488 nm laser or similar visible light. If necessary, it is recommended that you use Gsafe Green stain. Its sensitivity is the same as SYBR Green I, but its stability and reliability are far better than the latter. Plus can be used for both precast gel staining and post gel staining. Generally, post-staining can obtain more sensitive characteristics than pre-staining, and can exclude the possibility that the staining agent will have any effect on the separation of nucleic acid bands during the electrophoresis process. However, front dyeing is simpler and more economical than back dyeing because front dyeing does not require an additional coloring process and the amount of dye used is less. In addition, like GelGreen and EvaGreen, Plus has extremely low ability to induce mutations compared to EB or SYBR. Gsafe Red Plus nucleic acid dye, 10,000X in water is concentrated Gsafe Red Plus solution. When used for pre-dyeing, it can be diluted 10,000 times before use; when used for post-dyeing, it is recommended that you dilute it 3,300 times before use, see specific steps. |