您好,欢迎来到化工原料网! [登录] [免费注册]
化工原料网
位置:首页 > 产品库 > 4-Methylumbelliferyl-β-D-Galactoside
立即咨询
咨询类型:
     
*姓名:
*电话:
*单位:
Email:
*留言内容:
请详细说明您的需求。
*验证码:
 
4-Methylumbelliferyl-β-D-Galactoside
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
4-Methylumbelliferyl-β-D-Galactoside图片
CAS NO:6160-78-7
包装与价格:
包装价格(元)
250mg电议
500mg电议
1 g电议
5 g电议

产品介绍
4-Methylumbelliferyl-β-D-Galactoside 是 β-半乳糖苷酶的荧光底物,当它被切割时,会产生水溶性蓝色荧光香豆素荧光团,可以使用荧光酶或荧光计进行检测。
Cas No.6160-78-7
别名4-甲基伞形酮酰-beta-D-吡喃糖苷
Canonical SMILESCC(C1=CC=C(O[C@@H]2O[C@@H]([C@@H]([C@@H]([C@H]2O)O)O)CO)C=C1O3)=CC3=O
分子式C16H18O8
分子量338.3
溶解度50 mg/ml in DMSO (Need ultrasonic), Ethanol: slightly soluble, Methanol: slightly soluble
储存条件Store at -20℃
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述

4-Methylumbelliferyl-β-D-galactoside (MUG) is a fluorogenic substrate for β-galactosidase (β-gal).1Hydrolysis of MUG by β-gal releases the fluorescent moiety 4-MU, which displays a pH-dependent excitation maximum of 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively, and an emission maximum between 445-454 nm.2MUG has been used to detect β-gal activity in intact bacteria, yeast, and mammalian cells.1

1.Vidal-Aroca, F., Giannattasio, M., Brunelli, E., et al.One-step high-throughput assay for quantitative detection of β-galactosidase activity in intact Gram-negative bacteria, yeast, and mammalian cellsBiotechniques40(4)433-434(2006) 2.Zhi, H., Wang, J., Wang, S., et al.Fluorescent properties of hymecromone and fluorimetric analysis of hymecromone in compound dantong capsuleJ. Spectrosc.1(1)147128(2013)