| 包装 | 价格(元) |
| 1mg | 电议 |
| 5mg | 电议 |
| 10mg | 电议 |
| 50mg | 电议 |
Cell lines | HEK 293T |
Preparation Method | Cell viability was determined using RealTime-Glo MT Cell Viability Assay (Promega) after incubation of HEK 293T cells with individual luciferin substrates for 24 h at 37 ℃. Cell morphology was further evaluated using microscopy. |
Reaction Conditions | 24h 30 μM Diphenylterazine |
Applications | Compared to other substrates，As a novel luciferase substrate based on CTZ analogue, Diphenylterazine has bright red-shift bioluminescence. Moreover, Diphenylterazine alone yielded very little background and thus exhibited excellent signal-to-background ratios , Furthermore, Diphenylterazine elicited minimal cell toxicity at millimolar concentrations. |
Animal models | BALB/c mice |
Preparation Method | Bioluminescence imaging of intravenously injected HEK 293T cells：BALB/c mice are used and transfected with cells expressing biotinase gene by injecting cells into the tail vein of BALB/c mice. After the diminishing of the bioluminescence, 0.3 μmol Diphenylterazine is intraperitoneally injected. Mice are imaged with a 1-min exposure per frame over a course of 20 min. |
Dosage form | 0.3 μmol Diphenylterazine |
Applications | Diphenylterazine was used as an emerging luciferase reporter substrate in mice. The imaging of deep tissue targets in mice was evaluated by injecting luciferase gene expressing HEK 293T cells through the tail vein. First, Diphenylterazine as a substrate has no background in control mice, and when injected into mice transfected with the luciferase gene, the fluorescence emitted by Diphenylterazine can be detected, and the intensity of Diphenylterazine has increased significantly compared with other substrates. |
| 产品描述 | Diphenylterazine, an analog of CTZ, is a good luciferase substrate. Diphenylterazine is a bioluminescence agent. Diphenylterazine alone yields very little background, leading to excellent signal-to-background ratios. Furthermore, Diphenylterazine elicits minimal cell toxicity at millimolar concentrations. Diphenylterazine could be synthesized from inexpensive commercial reagents in two steps with excellent yields[1]. In cell experiments, we can see that Diphenylterazine has a lower signal-to-noise ratio than other substrates, and Diphenylterazine causes minimal cytotoxicity at milimolar concentrations. In contrast, other tested substrate induced cell death within the tested substrate concentration range. In the mouse experiment, the bioluminescence of the surface site, at 0.1 mM substrate concentration, Diphenylterazine brightness was higher than that of the control group. The same was true for imaging of deep tissue targets, and Diphenylterazine injection into untransfected BALB/c mice did not produce any background emission, and fluorescence was still detectable when injected intravenously into mice[2]. References: |
m.cnreagent.com