| CAS NO: | 57-71-6 |
| 包装 | 价格(元) |
| 10mM (in 1mL DMSO) | 电议 |
| 100g | 电议 |
| Storage | Store at -20°C |
| M.Wt | 101.1 |
| Cas No. | 57-71-6 |
| Formula | C4H7NO2 |
| Synonyms | BDM|Diacetyl Monoxime|NSC 660|NSC 116103 |
| Solubility | ≥5.25 mg/mL in DMSO; ≥60.2 mg/mL in H2O; ≥99.6 mg/mL in EtOH |
| Chemical Name | 2,3-butanedione, 2-oxime |
| Canonical SMILES | CC(/C(C)=N/O)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
2,3-Butanedione-2-monoxime is a myosin ATPase inhibitor.
Myosin, an ATPase, can convert chemical energy into directed movement and is regarded as a molecular motor. Myosin has various shapes and sizes. More than 11 myosin classes have been identified, and more will be found. The common feature of all of these molecules is a section close to the N terminus, which can be identified as a motor domain.
In vitro: 2,3-Butanedione-2-monoxime (BDM), a general probe of myosin function, was widely used in muscle research as a low-affinity but specific chemical phosphatase that could reversibly inhibit the myosin cross-bridge cycle. It was found that wild-type cells treated with BDM at 20 mM for around two generation times were smaller than untreated controls and showed a septation index about twice that observed in the absence of the inhibitor. Moreover, the organization of actin at the cell poles was disorganized in the presence of BDM, however, cells formed a cytokinetic actin ring. In addition, when nitrogen-starved stationary-phase cells were reinoculated into fresh medium in the presence of BDM, the time taken to repolarize the actin cytoskeleton and to resume the characteristic vegetative cell shape were both delayed substantially [1].
In vivo: So far, there is no animal in vivo data reported.
Clinical trial: So far, no clinical study has been conducted.
Reference:
[1] May KM, Wheatley SP, Amin V, Hyams JS. The myosin ATPase inhibitor 2,3-butanedione-2-monoxime (BDM) inhibits tip growth and cytokinesis in the fission yeast, Schizosaccharomyces pombe. Cell Motil Cytoskeleton. 1998;41(2):117-25.
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