(+)-Aphidicolin

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(+)-Aphidicolin

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

38966-21-1

包装与价格：

包装	价格(元)
1mg	电议
5mg	电议
25mg	电议

产品介绍

化学性质

Physical Appearance	A white to off-white solid
Storage	Store at -20°C
M.Wt	338.48
Cas No.	38966-21-1
Formula	C₂₀H₃₄O₄
Solubility	≥33.33 mg/mL in DMSO; insoluble in EtOH; insoluble in H2O
Chemical Name	(3R,4R,4aR,6aS,8R,9R,11aS,11bS)-4,9-bis(hydroxymethyl)-4,11b-dimethyltetradecahydro-8,11a-methanocyclohepta[a]naphthalene-3,9-diol
Canonical SMILES	O[C@@]1(CC[C@@]23[C@@]([H])(CC[C@]4([C@@](C)([C@@H](CC[C@@]43C)O)CO)[H])C[C@@H]1C2)CO
运输条件	蓝冰运输或根据您的需求运输。
一般建议	为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

Aphidicolin, a tetracyclic diterpenoid, is an antibiotic with novel structure produced by the mold Cephalosporium aphidicola. Aphidicolin is an inhibitor of DNA polymerase-α [1].

DNA polymerase-α is an enzyme complex involved in initiation of DNA replication and during synthesis of Okazaki fragments on the lagging strand. The DNA polymerase-α complex consists of 4 subunits: POLA1, POLA2, PRIM1, and PRIM2. POLA1 is the catalytic subunit. POLA2 is the regulatory subunit. PRIM1 and PRIM2 are the small and the large primase subunits [2].

Aphidicolin selectively inhibited the activity of DNA polymerase-α without activity for DNA polymerase-β and mitochondrial DNA polymerase. Aphidicolin prevented cell division in sea urchin embryos [1]. Aphidicolin was a potent inhibitor of cellular deoxyribonucleic acid synthesis. Aphidicolin strongly inhibited the growth of herpes simplex virus both in tissue culture and in the rabbit eye. Aphidicolin was active against iododeoxyuridine-resistant herpes virus [3]. Aphidicolin showed antiviral activity and inhibited the incorporation of thymidine into DNA of cultured human embryonic lung cells. Aphidicolin (15 μg/ml) reduced the activity of crude and partially purified DNA polymerases from the cytosol [4].

References:
[1] Ikegami S, TAGUCHI T, OHASHI M, et al. Aphidicolin prevents mitotic cell division by interfering with the activity of DNA polymerase-α[J]. Nature, 1978, 275(5679): 458-460.
[2] Lehman I R, Kaguni L S, Krause K H, et al. DNA polymerase alpha[J]. J. Biol. Chem, 1989, 264.
[3] Bucknall R A, Moores H, Simms R, et al. Antiviral effects of aphidicolin, a new antibiotic produced by Cephalosporium aphidicola[J]. Antimicrobial agents and chemotherapy, 1973, 4(3): 294-298.
[4] Ohashi M, Taguchi T, Ikegami S. Aphidicolin: a specific inhibitor of DNA polymerases in the cytosol of rat liver[J]. Biochemical and biophysical research communications, 1978, 82(4): 1084-1090.

试验操作

Cell experiment:[1]
Cell lines	HeLa cells
Reaction Conditions	0.03 ~ 30 μM aphidicolin for 12 h incubation
Applications	Treatment with low aphidicolin concentrations (0.03 and 0.3 μM) resulted in increasing normalized integrated nuclear intensity (NINI) values which corresponded to a block in G2/M phase, while high aphidicolin concentrations (3 and 30 μM) led to NINI values that corresponded to G1 phase. In addition, in HeLa cells pretreated with aphidicolin (0.01 ~ 10 μM), a dose-dependent decrease in percentage of Ebola virus (EBOV) infected cells was observed. Thus, aphidicolin was able to arrest cells in the G1/S boundary and restrict EBOV infection.
Note	The technical data provided above is for reference only.
	References: 1. Kota KP, Benko JG, Mudhasani R, et al. High content image based analysis identifies cell cycle inhibitors as regulators of Ebola virus infection. Viruses, 2012, 4(10): 1865-1877.