2,4-Diamino-6-hydroxypyrimidine is a specificGTP cyclohydrolase Iinhibitor (the rate-limiting enzyme in de novo pterin synthesis). 2,4-Diamino-6-hydroxypyrimidine blocks Tetrahydrobiopterin (BH4) synthesis and suppressesNOproduction^[1][2].

GTP cyclohydrolase I^[1]

2,4-Diamino-6-hydroxypyrimidine (DAHP) exhibits a dose-dependent reduction in NO production in both chicken peritoneal macrophages (PECs) and the avian MC29 virus-transformed macrophage cell line, HD11^[1].
2,4-Diamino-6-hydroxypyrimidine (DAHP; 5 mM; 24 hours) treatment significantly reduces both BH4 and VCAM-1 levels during the time course in HUVEC cells. 2,4-Diamino-6-hydroxypyrimidine reduces VCAM-1 and GTPCH protein synthesis at least partially via suppressing the NF-κB level in the nucleus of HUVEC^[2].
2,4-Diamino-6-hydroxypyrimidine (DAHP) is first identified as an inhibitor of the biopterin-dependent growth of the protozoanCrithidia fasciculate^[2].

When 2,4-Diamino-6-hydroxypyrimidine (DAHP) is administered for 4 days at the dose of 3 g/kg/day in mice, serotonin decreased in the duodenum and colon to approximately 46% and 40% of the control levels, respectively. 2,4-Diamino-6-hydroxypyrimidine causes a rapid decrease (T_1/2of less than 12 hr) in the BH4 levels in all tissues examined, except in the brain. The BH4 level returned to within the normal range less than 24 hr after cessation of the administration of 2,4-Diamino-6-hydroxypyrimidine^[3].

126.12

Solid

C₄H₆N₄O

56-06-4

2,4-二氨基-6-羟基嘧啶

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

DMSO : 16.67 mg/mL(132.18 mM;Need ultrasonic)

H₂O : 10 mg/mL(79.29 mM;Need ultrasonic)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： PBS

  Solubility: 6.5 mg/mL (51.54 mM); Clear solution; Need ultrasonic
• 2.

  请依序添加每种溶剂： 10% DMSO    40%PEG300   5%Tween-80   45% saline

  Solubility: ≥ 1.67 mg/mL (13.24 mM); Clear solution

  此方案可获得 ≥ 1.67 mg/mL (13.24 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 16.7 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

  将 0.9 g 氯化钠，完全溶解于 100 mL ddH2O 中，得到澄清透明的生理盐水溶液
• 3.

  请依序添加每种溶剂： 10% DMSO    90% (20%SBE-β-CDin saline)

  Solubility: ≥ 1.67 mg/mL (13.24 mM); Clear solution

  此方案可获得 ≥ 1.67 mg/mL (13.24 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 16.7 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

  将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
• 4.

  请依序添加每种溶剂： 10% DMSO    90%corn oil

  Solubility: ≥ 1.67 mg/mL (13.24 mM); Clear solution

  此方案可获得 ≥ 1.67 mg/mL (13.24 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

  以 1 mL 工作液为例，取 100 μL 16.7 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。