| 生物活性 | Ibuprofen ((±)-Ibuprofen) sodium is an orally active, selectiveCOX-1inhibitor with anIC50value of 13 μM. Ibuprofen sodium inhibits cell proliferation, angiogenesis, and induces cellapoptosis. Ibuprofen sodium is a nonsteroidal anti-inflammatory agent and a nitric oxide (NO) donor. Ibuprofen sodium can be used in the research of pain, swelling, inflammation,infection, immunology, cancers[1][2][5][8]. |
| IC50& Target[1] | COX-1 13 μM (IC50) | COX-2 370 μM (IC50) |
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体外研究
(In Vitro) | Ibuprofen sodium (24 h) inhibits COX-1 and COX-2 activity with IC50values of 13 μM and 370 μM[1].
Ibuprofen sodium (500 μM, 48 h) inhibits cell proliferation and angiogenesis, and induces apoptosis in AGS cells (Adenocarcinoma gastric cell line)[2].
Ibuprofen sodium (500 μM, 48 h) downregulates transcription of Akt, VEGF-A, PCNA, Bcl2, OCT3/4 and CD44 genes, but upregulates RNA levels of wild type P53 and Bax genes in AGS cell[2].
Ibuprofen sodium (500 μM, 24 h) restores microtubule reformation, microtubule-dependent intracellular cholesterol transport, and induces extension of microtubules to the cell periphery in both cystic fibrosis (CF) cell models and primary CF nasal epithelial cells[3].
Ibuprofen sodium (500 μM, 24 h) enhances UV-induced cell death in MCF-7 cells and MDA-MB-231 cells by a photosensitization process[4].
Cell Viability Assay[2] | Cell Line: | AGS cells | | Concentration: | 100-1000 μM | | Incubation Time: | 24 h, 48 h | | Result: | Inhibited AGS cell viability with IC50values of 630 μM (trypan blue staining, 24 h), 456 μM (neutral red assay, 24 h), 549 μM (trypan blue staining , 48 h) and 408 μM (neutral red assay, 48 h). |
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体内研究
(In Vivo) | Ibuprofen sodium (fed in animal feedings, 300 mg/kg, 14 days) reduces overall tumor growth and enhances anti-tumor immune characteristics without adverse autoimmune reactions in a model of postpartum breast cancer[5].
Ibuprofen sodium (subcutaneous injection, 60 mg/kg, every second day for 15 days) reduces the risk of neuropathy in a rat model of chronic Oxaliplatin-induced peripheral neuropathy[6].
Ibuprofen sodium (oral administration, 20 mg/kg, every 12 hours, 5 doses total) decreases muscle growth (average muscle fiber cross-sectional area) without affecting regulation of supraspinatus tendon adaptions to exercise[7].
Ibuprofen sodium (oral administration, 35 mg/kg, twice daily) attenuates the Inflammatory response topseudomonas aeruginosain a rat model of chronic pulmonary infection[8].
| Animal Model: | Syngeneic (D2A1) orthotopic Balb/c mouse model of PPBC (postpartum)[5] | | Dosage: | 300 mg/kg, daily for 14 days | | Administration: | Fed in animal feedings (added to pulverized standard chow and mixed dry, then mixed with water, made into chow pellets and dried thoroughly) | | Result: | Suppresed tumor growth, reduced presence of immature monocytes and increased numbers of T cells.
Enhanced Th1 associated cytokines as well as promoted tumor border accumulation of T cells. |
| Animal Model: | Oxaliplatin-induced peripheral neuropathy[6] | | Dosage: | 60 mg/kg, every second day for 15 days | | Administration: | Subcutaneous injection | | Result: | Lowered sensory nerve conduction velocity (SNCV). |
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| 中文名称 | 布洛芬钠盐；布洛芬钠盐；异丁苯丙酸钠盐；异丁洛芬钠盐 |
| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | 4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| 溶解性数据 | In Vitro: H2O : ≥ 100 mg/mL(438.10 mM) DMSO : 20.83 mg/mL(91.26 mM;ultrasonic and warming and heat to 60℃) *"≥" means soluble, but saturation unknown. 配制储备液 | 1 mM | 4.3810 mL | 21.9048 mL | 43.8097 mL | | 5 mM | 0.8762 mL | 4.3810 mL | 8.7619 mL | | 10 mM | 0.4381 mL | 2.1905 mL | 4.3810 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 |
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