质粒名称:CD109-bio-His
质粒编号:51862
质粒备注:Expresses full-length CD109 antigen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
载体名称:pTT3
载体大小:6618
完整质粒大小:10907
载体类型:哺乳动物表达
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:Top10
高拷贝/低拷贝:高拷贝
插入基因:CD109
基因别名:CD109 antigen precursor
物种来源:H. sapiens (human)
插入大小:4289
基因名称:CD109 (a.k.a. CPAMD7, p180, r150)
启动子:CMV
标签/融合蛋白:· rat CD4 d3+4 (C terminal on backbone)
· enzymatic biotinylation sequence (C terminal on backbone)
· His tag (C terminal on backbone)
克隆方法:限制性内切酶
5'克隆位点:NotI (not destroyed)
3'克隆位点:AscI (not destroyed)
5'测序引物:CMV-fwd
质粒图谱: