质粒名称:pET28a/Cas9-Cys
质粒编号:53261
质粒备注:Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine
载体名称:pET28a
载体大小:5369
完整质粒大小:9542
载体类型:原核表达
原核抗性:卡那霉素,50μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:未知
插入基因:Cas9-Cys
物种来源:S. pyogenes
基因突变:Additional Cysteine is added to the C-terminal region of Cas9
插入大小:4173
启动子:T7 Promoter
标签/融合蛋白:· 6X His tag (N terminal on backbone)
· HA epitope (C terminal on insert)
· NLS (C terminal on insert)
克隆方法:限制性内切酶
5'克隆位点:Not I (not destroyed)
3'克隆位点:Not I (not destroyed)
5'测序引物:TAA TAC GAC TCA CTA TAG GG
3'测序引物:GCT AGT TAT TGC TCA GCG
质粒图谱: