质粒名称:pLKO.1 hygro
质粒编号:24150
质粒备注:(Empty Backbone) 3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.
载体名称:pLKO.1 - TRC cloning vector (no stuffer)
载体大小:7500
载体类型:Mammalian Expression, Lentiviral, RNAi
筛选标记:潮霉素
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:Stbl3
高拷贝/低拷贝:高拷贝
插入基因:None
启动子:U6 for shRNA
克隆方法:限制性内切酶
5'克隆位点:AgeI (not destroyed)
3'克隆位点:EcoRI (not destroyed)
5'测序引物:LKO1_5
3'测序引物:SV40pA-R
质粒图谱: