质粒名称：pML107
质粒编号：67639
质粒备注：

Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

载体名称：pRSII425
载体大小：6841
完整质粒大小：12366
载体类型：Yeast Expression, CRISPR
筛选标记：LEU2

原核抗性：氨苄青霉素，100μg/mL
培养温度：37℃
克隆菌株：DH5alpha
高拷贝/低拷贝：高拷贝

插入基因：Cas9
物种来源：S. cerevisiae (budding yeast); Streptococcus pyogenes
插入大小：4151
启动子：pTDH3 (aka GAP promoter)

克隆方法：限制性内切酶
5'克隆位点：EcoRI (not destroyed)
3'克隆位点：XhoI (not destroyed)
5'测序引物：T7

质粒图谱：