质粒名称:TLCV2
质粒编号:87360
质粒备注:LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.
载体名称:lentiCRISPR v2
载体大小:16733
载体类型:Mammalian Expression, Lentiviral, CRISPR ; Doxycycline inducible; eGFP reporter
筛选标记:嘌呤霉素
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:NEB Stable
高拷贝/低拷贝:未知
插入基因:Cas9-2A-eGFP
启动子:Tight TRE promoter
克隆方法:未知
5'测序引物:LNCX [5'-AGCTCGTTTAGTGAACCGTCAGATC-3']
3'测序引物:EF1a-R [5'CACGGCGACTACTGCACTTA-3']
质粒图谱: