质粒名称:pMIG-hNFATc2/C - AVITEV
质粒编号:74058
质粒备注:retroviral expression plasmid for human NFATc2/C with C-terminal BirA-biotinylation signal and TEV celavage site
载体名称:pMSCV-IRES-GFP (pMIG)
载体大小:7200
完整质粒大小:9935
载体类型:哺乳动物表达,逆转录
筛选标记:GFP
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:NEB Stable
高拷贝/低拷贝:未知
插入基因:human NFATc2, isoform C
基因别名:NFAT1
基因别名:nuclear factor of activated T-cells 2
物种来源:H. sapiens (human)
基因突变:silent mutation A1723C in the sequence of NM_173091.3 (A1503C position in ORF of the vector)
插入大小:2700
基因名称:NFATC2 (a.k.a. JCOSL, NFAT1, NFATP)
启动子:pMSCV-LTRs
克隆方法:限制性内切酶
5'克隆位点:SacII (not destroyed)
3'克隆位点:SnaB1, Sgf1 (not destroyed)
5'测序引物:CGTTCGACCCCGCCTCGATCC
3'测序引物:AGCTTGATATCGAATTCCG
质粒图谱: