常用荧光染料的激发和发射波长
这是一个用于流式细胞术或荧光显微镜的荧光色素的一些特性的表。Yarebio在组内,大致按激发波长的顺序排列(家族除外)。峰值激发和发射波长通常随探测器所处的环境而变化。所以需要自行确认所选染料的激发光谱和发射光谱。另外,下表显示的颜色只是一个非常粗略的近似值,并且不同显示设备会有一定色差!
Probe | Ex (nm) | Em (nm) | MW | Notes |
---|
Reactive and conjugated probes |
Hydroxycoumarin | 325 | 386 | 331 | Succinimidyl ester |
Aminocoumarin | 350 | 445 | 330 | Succinimidyl ester |
Methoxycoumarin | 360 | 410 | 317 | Succinimidyl ester |
Cascade Blue | (375);401 | 423 | 596 | Hydrazide |
Pacific Blue | 403 | 455 | 406 | Maleimide |
Pacific Orange | 403 | 551 |
|
|
Lucifer yellow | 425 | 528 |
|
|
NBD | 466 | 539 | 294 | NBD-X |
R-Phycoerythrin (PE) | 480;565 | 578 | 240 k |
|
PE-Cy5 conjugates | 480;565;650 | 670 |
| aka Cychrome, R670, Tri-Color, Quantum Red |
PE-Cy7 conjugates | 480;565;743 | 767 |
|
|
Red 613 | 480;565 | 613 |
| PE-Texas Red |
PerCP | 490 | 675 |
| Peridinin chlorphyll protein |
TruRed | 490,675 | 695 |
| PerCP-Cy5.5 conjugate |
FluorX | 494 | 520 | 587 | (GE Healthcare) |
Fluorescein | 495 | 519 | 389 | FITC; pH sensitive |
BODIPY-FL | 503 | 512 |
|
|
TRITC | 547 | 572 | 444 | TRITC |
X-Rhodamine | 570 | 576 | 548 | XRITC |
Lissamine Rhodamine B | 570 | 590 |
|
|
Texas Red | 589 | 615 | 625 | Sulfonyl chloride |
Allophycocyanin (APC) | 650 | 660 | 104 k |
|
APC-Cy7 conjugates | 650;755 | 767 |
| PharRed |
Alexa Fluor dyes [antibody conjugates] (Molecular Probes) |
Alexa Fluor 350 | 343 | 442 | 410 |
|
Alexa Fluor 405 | 401 | 421 | 1028 |
|
Alexa Fluor 430 | 434 | 540 | 702 |
|
Alexa Fluor 488 | 499 | 519 | 643 | QY 0.92 |
Alexa Fluor 500 | 503 | 525 | 700 |
|
Alexa Fluor 514 | 517 | 542 | 714 |
|
Alexa Fluor 532 | 530 | 555 | 724 | QY 0.61 |
Alexa Fluor 546 | 561 | 572 | 1079 | QY 0.79 |
Alexa Fluor 555 | 553 | 568 | 1250 | QY 0.1 |
Alexa Fluor 568 | 579 | 603 | 792 | QY 0.69 |
Alexa Fluor 594 | 591 | 618 | 820 | QY 0.66 |
Alexa Fluor 610 | 610 | 629 | 1285 |
|
Alexa Fluor 633 | 632 | 648 | 1200 |
|
Alexa Fluor 647 | 652 | 668 | 1300 | QY 0.33 |
Alexa Fluor 660 | 663 | 691 | 1100 |
|
Alexa Fluor 680 | 680 | 702 | 1150 |
|
Alexa Fluor 700 | 696 | 719 | 1400 |
|
Alexa Fluor 750 | 752 | 776 | 1300 |
|
Alexa Fluor 790 | 782 | 804 | 1750 |
|
Cy Dyes |
Cy2 | 489 | 506 | 714 | QY 0.12 |
Cy3 | (512);550 | 570;(615) | 767 | QY 0.15 |
Cy3B | 558 | 572;(620) | 658 | QY 0.67 |
Cy3.5 | 581 | 594;(640) | 1102 | QY 0.15 |
Cy5 | (625);650 | 670 | 792 | QY 0.28 |
Cy5.5 | 675 | 694 | 1128 | QY 0.23 |
Cy7 | 743 | 767 | 818 | QY 0.28 |
DyLight dyes (Pierce) |
DyLight 350 | 353 | 432 |
|
|
DyLight 405 | 400 | 420 |
|
|
DyLight 488 | 493 | 518 |
|
|
DyLight 549 | 562 | 576 |
|
|
DyLight 594 | 593 | 618 |
|
|
DyLight 633 | 638 | 658 |
|
|
DyLight 649 | 654 | 673 |
|
|
DyLight 680 | 692 | 712 |
|
|
DyLight 750 | 752 | 778 |
|
|
DyLight 800 | 777 | 794 |
|
|
Nucleic acid probes |
Hoechst 33342 | 343 | 483 | 616 | AT-selective |
DAPI | 345 | 455 |
| AT-selective |
Hoechst 33258 | 345 | 478 | 624 | AT-selective |
SYTOX Blue | 431 | 480 | ~400 | DNA |
Chromomycin A3 | 445 | 575 |
| CG-selective |
Mithramycin | 445 | 575 |
|
|
YOYO-1 | 491 | 509 | 1271 |
|
Ethidium Bromide | 493 | 620 | 394 |
|
Acridine Orange | 503 | 530/640 |
| DNA/RNA |
SYTOX Green | 504 | 523 | ~600 | DNA |
TOTO-1, TO-PRO-1 | 509 | 533 |
| Vital stain, TOTO: Cyanine Dimer TO-PRO: Cyanine Monomer |
Thiazole Orange | 510 | 530 |
|
|
Propidium Iodide (PI) | 536 | 617 | 668.4 |
|
LDS 751 | 543;590 | 712;607 | 472 | DNA (543ex/712em), RNA (590ex/607em) |
7-AAD | 546 | 647 |
| 7-aminoactinomycin D, CG-selective |
SYTOX Orange | 547 | 570 | ~500 | DNA |
TOTO-3, TO-PRO-3 | 642 | 661 |
|
|
DRAQ5 | 647 | 681,697 | 413 | (Biostatus) (usable excitation down to 488) |
Cell function probes |
Indo-1 | 361/330 | 490/405 | 1010 | AM ester. Low/High Ca++, |
Fluo-3 | 506 | 526 | 855 | AM ester. pH > 6 |
DCFH | 505 | 535 | 529 | 2'7'Dichorodihydrofluorescein, oxidized form |
DHR | 505 | 534 | 346 | Dihydrorhodamine 123, oxidized form, light catalyzes oxidation |
SNARF | 548/579 | 587/635 |
| pH 6/9 |
Fluorescent Proteins |
| QY | BR | PS | Source |
|
---|
Y66H | 360 | 442 |
|
|
|
|
|
|
Y66F | 360 | 508 |
|
|
|
|
|
|
EBFP | 380 | 440 |
| 0.18 | 9 |
| Addgene | monomer |
EBFP2 | 383 | 448 |
| 0.56 | 18 |
| Addgene | monomer |
Azurite | 383 | 447 |
| 0.55 | 14 |
|
| monomer |
GFPuv | 385 | 508 |
|
|
|
|
|
|
T-Sapphire | 399 | 511 |
| 0.60 | 26 | 25 |
| weak dimer |
TagBFP | 402 | 457 | 26k | 0.63 | 33 | ++ | Evrogen | monomer |
Cerulean | 433 | 475 |
| 0.62 | 27 | 36 |
| weak dimer |
mCFP | 433 | 475 |
| 0.40 | 13 | 64 |
| monomer |
ECFP | 434 | 477 |
| 0.15 | 3 |
|
|
|
CyPet | 435 | 477 |
| 0.51 | 18 | 59 |
| weak dimer |
Y66W | 436 | 485 |
|
|
|
|
|
|
dKeima-Red | 440 | 616 |
| 0.31 | 8 |
| MBL | dimer |
mKeima-Red | 440 | 620 |
| 0.24 | 3 |
| MBL | monomer |
TagCFP | 458 | 480 |
| 0.57 | 29 |
| Evrogen | dimer |
AmCyan1 | 458 | 489 |
| 0.75 | 29 |
| Clontech | tetramer |
mTFP1 (Teal) | 462 | 492 |
| 0.85 | 54 |
|
| dimer |
S65A | 471 | 504 |
|
|
|
|
|
|
Midoriishi-Cyan | 472 | 495 |
| 0.9 | 25 |
| MBL | dimer |
Wild Type GFP | 396,475 | 508 | 26k | 0.77 | 16 |
|
|
|
S65C | 479 | 507 |
|
|
|
|
|
|
TurboGFP | 482 | 502 | 26 k | 0.53 | 37 |
| Evrogen | dimer |
TagGFP | 482 | 505 | 27k | 0.59 | 34 | ++ | Evrogen | monomer |
TagGFP2 | 483 | 506 | 27k | 0.6 | 34 | ++ | Evrogen | monomer |
AcGFP1 | 484 | 510 | 27k | 0.82 | 27 |
| Clontech |
|
S65L | 484 | 510 |
|
|
|
|
|
|
Emerald | 487 | 509 |
| 0.68 | 39 | 0.69 | Invitrogen | weak dimer |
S65T | 488 | 511 |
|
|
|
|
|
|
EGFP | 488 | 507 | 26k | 0.60 | 34 | 174 | Addgene | weak dimer |
Azami-Green | 492 | 505 |
| 0.74 | 41 |
| MBL | tetramer (monomeric available) |
ZsGreen1 | 493 | 505 | 105k | 0.91 | 40 |
| Clontech | tetramer |
Dronpa-Green | 503 | 518 |
| 0.85 | 81 |
| MBL | photoswitchable |
TagYFP | 508 | 524 | 27k | 0.62 | 47 |
| Evrogen | monomer |
EYFP | 514 | 527 | 26k | 0.61 | 51 | 60 |
| weak dimer |
Topaz | 514 | 527 |
| 0.60 | 57 |
|
| monomer |
Venus | 515 | 528 |
| 0.57 | 53 | 15 |
| weak dimer |
mCitrine | 516 | 529 |
| 0.76 | 59 | 49 |
| monomer |
YPet | 517 | 530 |
| 0.77 | 80 | 49 |
| weak dimer |
TurboYFP | 525 | 538 | 26 k | 0.53 | 56 |
| Evrogen | dimer |
PhiYFP | 525 | 537 | 26.8 k | 0.40 | 52 | ++ | Evrogen | weak dimer |
PhiYFP-m | 525 | 537 | 26.8 k | 0.39 | 48 | ++ | Evrogen | monomer |
ZsYellow1 | 529 | 539 |
| 0.65 | 13 |
| Clontech | tetramer |
mBanana | 540 | 553 |
| 0.70 | 4 |
| Clontech | monomer |
Kusabira-Orange | 548 | 559 |
| 0.60 | 31 |
| MBL | monomer |
mOrange | 548 | 562 |
| 0.69 | 49 | 9 |
| monomer |
mOrange2 | 549 | 565 |
| 0.60 | 35 |
| Clontech | monomer |
mKO | 548 | 559 |
| 0.60 | 31 | 122 |
| monomer |
TurboRFP | 553 | 574 | 26 k | 0.67 | 62 |
| Evrogen | dimer |
tdTomato | 554 | 581 |
| 0.69 | 95 | 98 | Clontech | tandem dimer |
DsRed-Express2 | 554 | 591 |
| 0.42 | 15 |
| Clontech |
|
TagRFP | 555 | 584 | 27k | 0.48 | 48 |
| Evrogen | monomer |
DsRed monomer | 557 | 592 | ~28k | 0.1 | 3.5 | 16 | Clontech | monomer |
DsRed2 ("RFP") | 563 | 582 | ~110k | 0.55 | 24 |
| Clontech |
|
mStrawberry | 574 | 596 |
| 0.29 | 26 | 15 | Clontech | monomer |
TurboFP602 | 574 | 602 | 26 k | 0.35 | 26 |
| Evrogen | dimer |
AsRed2 | 576 | 592 | ~110k | 0.21 | 13 |
| Clontech | tetramer |
mRFP1 | 584 | 607 | ~30k | 0.25 |
|
| Tsien lab | monomer |
J-Red | 584 | 610 |
| 0.20 | 8.8 | 13 |
| dimer |
mCherry | 587 | 610 |
| 0.22 | 16 | 96 | Clontech | monomer |
HcRed1 | 588 | 618 | ~52k | 0.03 | 0.6 |
| Clontech | dimer |
mKate2 | 588 | 633 | 26k | 0.40 | 25 | + | Evrogen | monomer |
Katushka (TurboFP635) | 588 | 635 | 26k | 0.34 | 22 | ++ | Evrogen | dimer |
mKate (TagFP635) | 588 | 635 |
| 0.30 | 15 |
| Evrogen | monomer |
TurboFP635 | 588 | 635 | 26 k | 0.34 | 22 |
| Evrogen | dimer |
mPlum | 590 | 649 |
| 0.10 | 4.1 | 53 | Clontech |
|
mRaspberry | 598 | 625 |
| 0.15 | 13 |
| Clontech | monomer; faster photobleach than mPlum |
mNeptune | 600 | 650 |
| 0.20 | 13 |
| Tsien Lab | monomer |
E2-Crimson | 611 | 646 |
| 0.23 | 29 |
| Clontech |
|
Other probes |
Monochlorobimane | 380 | 461 | 226 | Glutathione probe |
Calcein | 496 | 517 | 623 | pH > 5 |
HyPer | 420/500 | 516 |
| Hydrogen peroxide sensor |
Legend:
Ex: Peak excitation wavelength (nm)
Em: Peak emission wavelength (nm)
MW: Molecular weight
QY: Quantum yield
BR: Brightness; Extinction coefficient * Quantum yield / 1000
PS: Photostability; time to 50% brightness (sec)