癌胚抗原单克隆抗体

商品属性

产品介绍
英文名称CEACAM5 Mouse mAb

中文名称癌胚抗原单克隆抗体

别    名Carcinoembryonic antigen; Carcinoembryonic antigen related cell adhesion molecule 5; Carcinoembryonic antigen-related cell adhesion molecule 5; CD66e; CD66e antigen; CEACAM5; CEAM5_HUMAN; DKFZp781M2392; Meconium antigen 100.  

研究领域肿瘤  细胞类型标志物  

抗体来源Mouse

克隆类型Monoclonal

克 隆 号3C12

交叉反应Human

产品应用WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量77 kDa

检测分子量180-200

细胞定位细胞膜

性    状Liquid

浓    度1mg/ml

免 疫 原Recombinant human CEACAM5 protein

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

产品介绍CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glyco-protein (PSG 1-PSG 11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/ CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria.

靶点与功能

癌胚抗原（CEA，CEACAM5）是胚胎期胃肠道上皮细胞表达的糖蛋白，分子量约180-200kDa，含70%糖基化结构。它的核心临床价值在于：

肿瘤诊断：结直肠癌、胃癌、肺癌等上皮源性肿瘤中高表达，血清CEA水平＞5ng/mL提示恶性风险（正常参考值≤3.4ng/mL）；

疗效监测：术后CEA持续升高提示肿瘤复发或转移，灵敏度优于影像学检查；

实验研究：通过免疫组化或ELISA检测CEA表达，辅助肿瘤细胞系鉴定和药物筛选。

实验应用关键操作

ELISA定量检测（血清样本）：

双抗体夹心法：包被抗体（捕获抗体）1μg/mL 4℃包被过夜，样本经1:10稀释后37℃孵育2小时，检测抗体（生物素标记）1:2000稀释，HRP-链霉亲和素显色，450nm读取OD值；

关键技巧：标准曲线范围设为0.1-100ng/mL，使用含1% BSA的PBS作为稀释液，避免非特异性结合。

IHC-P组织染色（结直肠癌样本）：

抗原修复：柠檬酸钠缓冲液（pH6.0）微波修复20分钟（95℃），自然冷却至室温；

阳性定位：肿瘤细胞胞膜/胞质呈棕黄色颗粒状阳性，正常肠黏膜上皮细胞阴性或弱阳性；

对照设置：用CEA阳性结肠癌组织作阳性对照，正常肺组织作阴性对照。

WB验证（LS174T结肠癌细胞系）：

样本处理：RIPA裂解液（含1mM PMSF）冰上裂解30分钟，12000g离心取上清，上样量50μg总蛋白；

关键技巧：使用8% SDS-PAGE凝胶（分离大分子量蛋白），一抗1:1000稀释（5%脱脂奶粉+TBST），4℃孵育过夜，曝光30秒可见180-220kDa条带。

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