环腺苷酸应答元件结合蛋白转录共激活因子TORC1抗体

商品属性

产品介绍
英文名称CRTC1 Rabbit pAb

中文名称环腺苷酸应答元件结合蛋白转录共激活因子TORC1抗体

别    名CRTC1_HUMAN; CREB-regulated transcription coactivator 1; CREB regulated transcription coactivator 1; KIAA0616; MECT1; TORC1; WAMTP1; Mucoepidermoid carcinoma translocated protein 1; Transducer of regulated cAMP response element-binding protein 1(TORC-1; Transducer of CREB protein 1);  

抗体来源Rabbit

克隆类型Polyclonal

克 隆 号

交叉反应Human,Mouse,Rat (predicted: Pig,Cow,Dog,Horse)

产品应用WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量67 kDa

检测分子量

细胞定位细胞核 细胞浆

性    状Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from mouse CRTC1: 401-500/630

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

产品介绍which activates transcription through both consensus and variant cAMP response element (CRE) sites. MECT1 does not appear to modulate CREB1 DNA-binding activity but enhances the interaction of CREB1 with TAF4/TAFII-130. MECT1 translocates with MAML2 (MasterMind-Like Protein 2) to yield a fusion oncogene: t(11;19) (q21;p13). This translocation occurs in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas. The novel fusion product that results disrupts the Notch signaling pathway. The fusion protein consists of the N-terminus of MECT1 joined to the C-terminus of MAML2. The reciprocal fusion protein consisting of the N-terminus of MAML2 joined to the C-terminus of MECT1 has been detected in a small number of mucoepidermoid carcinomas. Multiple isoforms have been reported for the MECT1 protein.

靶点与功能

CRIM1（Cysteine-Rich Transmembrane BMP Regulator 1）是富含半胱氨酸的跨膜蛋白，分子量约100-120kDa，通过与BMP（骨形态发生蛋白）家族成员（如BMP4、BMP7）结合，调控其加工与细胞表面递送，参与中枢神经系统发育（尤其是运动神经元分化）、血管生成及肾脏形态发生。其表达异常与神经管缺陷、肾脏疾病及肿瘤血管生成密切相关，是神经发育与肿瘤微环境研究的重要标志物。

实验操作关键要点

WB优化（神经组织样本）：

样本处理：使用含蛋白酶抑制剂（1mM PMSF） 的RIPA裂解液冰上裂解30分钟，12000g离心取上清；若检测磷酸化修饰，需添加磷酸酶抑制剂；

电泳条件：8% SDS-PAGE凝胶（因分子量较大），恒压80V浓缩胶、120V分离胶，目标条带约106kDa；

抗体孵育：单克隆抗体推荐1:1500稀释（5% BSA+TBST），4℃孵育过夜，HRP二抗（山羊抗兔）1:5000室温孵育1小时。

IHC-P注意事项（脑组织切片）：

抗原修复：采用柠檬酸钠缓冲液（pH6.0） 微波修复15分钟（95℃），自然冷却至室温；

阳性定位：细胞膜/胞质呈棕黄色颗粒状阳性，在胚胎神经管、肾小球足细胞中高表达；

封闭处理：5%正常山羊血清+0.3% Triton X-100室温封闭45分钟，降低非特异性结合。

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