商品属性

产品介绍Arginase I which is expressed almost exclusively in the liver, catalyzes the conversion of arginine to ornithine and urea . The human arginase I gene, which maps to chromosome 6q23, encodes a 322 amino acid protein. Arginase I exists as a homotrimeric protein and contains a binuclear manganese cluster. Arginase II catalyzes the same reaction as arginase I, but differs in its tissue specificity and subcellular location. Specifically, arginase II localizes to the mitochondria. Arginase II is expressed in non-hepatic tissues, with the highest levels of expression in the kidneys, but, unlike arginase I, is not expressed in liver. The human arginase II gene, which maps to chromosome 14q24.1-q24.3, encodes a 354 amino acid protein. In addition, arginase II contains a putative amino-terminal mitochondrial localization sequence.

商品详情

英文名称Arginase 1 Mouse mAb

中文名称精氨酸酶1单克隆抗体

别    名liver Arginase; ARG 1; ARG1; ARGI1_HUMAN; Arginase1; Arginase liver; Arginase type I; Arginase I; ArginaseI; Arginase-1; Arginase1; Liver type arginase; Liver-type arginase; Type I arginase.  

抗体来源Mouse

克隆类型Monoclonal

克 隆 号7A15

交叉反应Human

产品应用IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量35 kDa

检测分子量

性    状Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human Arginase 1

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

应用数据

推荐稀释比例：

WB: 1:100–2000

IHC（石蜡切片）: 1:100–500（需抗原修复）

Flow Cytometry: 1:20–100

ELISA: 1:500–10000

交叉反应性：人、小鼠、大鼠、狗、猪、牛、兔、羊等。

质量控制

经WB、IHC、流式等多种实验验证。

实验操作建议

石蜡切片处理：建议使用柠檬酸钠缓冲液（pH 6.0）煮沸修复抗原15分钟，3%过氧化氢封闭内源性过氧化物酶。

二抗选择：若一抗为兔源，需选用抗兔二抗（如山羊抗兔IgG-FITC）。

流式细胞术：建议70%甲醇固定细胞，封闭非特异性结合后孵育一抗（30分钟，室温）。

注意事项

不同批次抗体可能存在浓度差异，建议**使用时进行梯度稀释优化。

石蜡切片需注意抗原修复条件，避免假阴性结果。

长期保存需分装，避免反复冻融导致效价降低。

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