商品属性

产品介绍The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known. [provided by RefSeq, Mar 2010].

商品详情

英文名称ATP1B1 Recombinant Rabbit mAb

中文名称钠钾ATP酶蛋白b1重组兔单抗

别    名beta 1 Sodium Potassium ATPase; Adenosinetriphosphatase; AT1B1_HUMAN; ATP 1B; ATP1B; ATP1B1; ATPase Na+/K+transporting beta 1 polypeptide; Atpb 1; ATPBS; beta 1 subunit of Na(+) K(+) ATPase; Na K ATPase beta 1 polypeptide; Na+/K+ATPase beta 1 subunit; Sodium/potassium dependent ATPase beta 1 subunit; Sodium/potassium transporting ATPase beta 1 chain; Sodium/potassium transporting ATPase subunit beta 1; Sodium/potassium-dependent ATPase subunit beta-1; Sodium/potassium-transporting ATPase subunit beta-1.  

抗体来源Rabbit

克隆类型Recombinant

克 隆 号2B9

交叉反应Human,Mouse,Rat

产品应用IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量35 kDa

检测分子量

细胞定位细胞膜

性    状Liquid

浓    度1mg/ml

免 疫 原A synthesized peptide derived from human ATP1B1: 261-303

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

靶点与功能

钠钾ATP酶β1亚基（ATP1B1）是P型ATP酶家族的跨膜糖蛋白（分子量约35-38kDa），核心功能与研究价值包括：

酶活性调控枢纽：与α亚基形成异源二聚体，通过胞外结构域的糖基化修饰（如Asn158位点）稳定α亚基构象，提升Na⁺/K⁺转运效率（β1缺失会导致酶活性降低60%以上）；

细胞极性标志物：在肾小管上皮细胞顶膜高表达，参与钠水重吸收，其表达异常与慢性肾病（CKD）患者尿钠排泄增加密切相关；

神经疾病机制：在星形胶质细胞中通过与水通道蛋白4（AQP4）共定位，调控脑水肿时的水-离子平衡，阿尔茨海默病模型中β1亚基下调会加剧神经元去极化。

实验操作关键要点

WB细胞膜蛋白检测：

样本处理：使用膜蛋白提取试剂盒分离肾脏/脑组织膜组分，裂解液添加蛋白酶抑制剂（1mM PMSF） 和磷酸酶抑制剂，冰上操作30分钟；

电泳条件：10% SDS-PAGE凝胶，恒压80V浓缩胶、120V分离胶，电泳90分钟（确保35-38kDa条带与杂蛋白分离）；

抗体孵育：抗体1:1000稀释（5% BSA+TBST），4℃摇床孵育过夜，HRP二抗（山羊抗兔）1:5000室温孵育1小时，曝光时间30-60秒。

IHC-P肾脏组织染色：

抗原修复：柠檬酸盐缓冲液（pH6.0）微波修复15分钟（石蜡切片），肾小管上皮细胞细胞膜呈棕黄色阳性，近端小管信号强于远端小管；

阳性对照：正常大鼠肾脏皮质组织（近曲小管刷状缘膜阳性）；

疾病模型：缺血再灌注肾损伤模型中，β1亚基表达下调50%，与血肌酐水平正相关（R²=0.68）。

IF细胞共定位研究：

与α1亚基抗体共染（钠钾ATP酶全酶标记），β1（绿色）与α1（红色）在细胞膜共定位形成“网格状分布”，共定位系数>0.8提示亚基组装正常；

与AQP4抗体共染（脑星形胶质细胞），可见二者在血管周足突共表达，脑水肿模型中共定位消失。

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