商品属性

产品介绍The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008].

商品详情

英文名称Bax Recombinant Rabbit mAb

中文名称Bax重组兔单抗

别    名apoptosis regulator BAX; Apoptosis regulator BAX cytoplasmic isoform beta; Apoptosis regulator BAX membrane isoform alpha; Bax isoform psi; BAX protein cytoplasmic isoform delta; Bax protein cytoplasmic isoform delta. antibody Bax protein cytoplasmic isoform gamma; Bax zeta; Bax-protein; Bcl-2-like protein 4; BCL2 associated X protein; BCL2L4; BAX_HUMAN; Bcl2-L-4.  

研究领域细胞生物  信号转导  细胞凋亡  

抗体来源Rabbit

克隆类型Recombinant

克 隆 号3C3

交叉反应Human,Mouse,Rat

产品应用WB=1:1000-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量21 kDa

检测分子量

细胞定位细胞浆 细胞膜

性    状Liquid

浓    度1mg/ml

免 疫 原A synthesized peptide derived from human BAX: 5-40

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

标记与检测

标记选项：FITC、HRP、PE-Cy3、Alexa Fluor系列（488/555/647等）

适用技术：Western Blot、免疫组化（IHC）、流式细胞术（FACS）、免疫荧光（IF）。

保存条件：

液态：-20℃保存；冻干粉：常温运输，复溶后分装冻存。

实验建议

使用浓度：

推荐起始浓度：1mg/ml（需根据实验体系优化，如WB常用1:1000稀释）。

二抗选择：

若一抗为兔源单抗，建议使用抗兔IgG-HRP或抗兔IgG-FITC二抗。

质量控制：

经ELISA、WB等多实验验证，部分品牌承诺无效退款。

注意事项

避免反复冻融，分装后保存。

冻干粉需用PBS或专用缓冲液复溶，离心后使用。

不同种属样本需验证交叉反应性。

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