商品属性

产品介绍The Bcl-2 gene was isolated at the chromosomal breakpoint of t(14;18)-bearing follicular B cell lymphomas(1,2).Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal (3,5).Bcl-2 appears to function in several subcellular locations yet lacks any known motifs that would confer insight into its mechanism of action (6,7).A more recently identified protein,designated Bax p21(i.e., Bcl-associated X protein ),has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2(8). Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activty of Bcl-2(8).

商品详情

英文名称Bcl-2 Mouse mAb

中文名称Bcl-2单克隆抗体

别    名Apoptosis regulator Bcl 2; Apoptosis regulator Bcl2; AW986256; B cell CLL/lymphoma 2; B cell leukemia/lymphoma 2; B cell lymphoma 2; Bcl 2; Bcl-2; Bcl2; BCL2 protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; Leukemia/lymphoma, B-cell, 2; Oncogene B-cell leukemia 2; BCL2_HUMAN.  

研究领域肿瘤  心血管  细胞生物  信号转导  细胞凋亡  新陈代谢  线粒体  

抗体来源Mouse

克隆类型Monoclonal

克 隆 号3F12

交叉反应Human,Rat (predicted: Mouse,Rabbit,Pig,Sheep,Cow,Dog,GuineaPig,Horse)

产品应用WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量26 kDa

检测分子量

细胞定位细胞核 细胞浆 细胞膜 线粒体

性    状Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human Bcl-2: 51-150/239

亚    型IgG1

纯化方法affinity purified by Protein G

缓 冲 液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

应用推荐稀释比

WB: 1:100–400

ICC/IHC: 1:100–500

ELISA: 需根据实验体系优化

实验原理（以ELISA为例）

采用双抗体夹心法：

包被：微孔板预包被抗LDLR抗体。

结合：样本中的LDLR与固相抗体结合后，加入HRP标记的二抗形成复合物。

显色：TMB底物在HRP催化下显色，颜色深度与LDLR浓度正相关。

检测：450 nm波长下测定OD值，通过标准曲线定量。

试剂盒组成（示例）

预包被微孔板（48孔或96孔配置）

标准品、HRP标记抗体、TMB显色液

洗涤缓冲液、终止液

说明书及质检报告

注意事项

样本类型：人/小鼠血清、血浆或细胞培养上清（需避免反复冻融）。

保存条件：抗体-20℃长期保存；试剂盒组分按标签要求存放（部分需4℃避光）。

交叉反应：需验证与其他物种LDLR的交叉性。

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