商品属性

产品介绍POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. The POU dimer from the OCT1 gene formed on the palindromic OCT factor recognition element, or PORE (ATTTGAAATGCAAAT), could recruit the transcriptional coactivator OBF1. Studies of tissue-specific expression of immunoglobulin promoters demonstrate the importance of an octamer, ATTTGCAT, and the proteins that bind to it. This is a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. Oct-1 encodes one protein, NF-A1, which is found in nuclear extracts from all cell types and thus is not specific to lymphoid cells as is the protein NF-A2, which is encoded by Oct-2. A novel protein designated Bob 1 (B cell Oct binding protein 1), alternatively called OBF-1, specifically interacts with Oct-1 and Oct-2, enhancing their transcriptional efficacy. Bob 1 is expressed at highest levels in spleen and peripheral blood leukocytes and represents an Oct co-factor capable of conferring cell-specific activation of Oct-1 and Oct-2. Although having no intrinsic capacity for DNA binding, Bob 1 associates tightly with the octamer motif in the presence of Oct-1 and/or Oct-2. The gene which encodes Bob 1 maps to human chromosome 11q23.1.

商品详情

英文名称BOB1 Mouse mAb

中文名称B细胞特异性转录因子OBF-1单克隆抗体

别    名B-cell oct-binding protein 1; B-cell-specific coactivator OBF-1; POU domain class 2-associating factor 1; POU2AF1; OCT-binding factor 1; BOB-1; OCA-B. OBF1; OCA-B; OBF1_HUMAN; BOB.1.  

抗体来源Mouse

克隆类型Monoclonal

克 隆 号5G9

交叉反应Human

产品应用IHC-P=1:200-500,IHC-F=1:200-500,IF=1:200-500

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量28 kDa

检测分子量

性    状Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human BOb-1

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

靶点与功能

OBF-1（POU2AF1基因编码）是B细胞特异性转录共激活因子（分子量约28kDa），核心功能与研究价值包括：

B细胞发育调控：通过结合POU结构域转录因子（如Oct-1/2），协同激活免疫球蛋白（Ig）基因启动子和增强子，是B细胞成熟、抗体类别转换的关键调控因子（OBF-1敲除小鼠B细胞数量减少50%，无生发中心形成）；

淋巴瘤标志物：在弥漫大B细胞淋巴瘤（DLBCL）、滤泡性淋巴瘤（FL） 中高表达，与CD10、Bcl-6联合检测可辅助生发中心来源淋巴瘤的分型；

免疫应答机制：参与T细胞依赖的B细胞活化，调控IL-4诱导的IgE类别转换，与过敏性疾病和自身免疫病密切相关。

实验操作关键要点

IHC-P淋巴瘤分型：

抗原修复：柠檬酸盐缓冲液（pH6.0）微波修复20分钟（石蜡切片），DLBCL组织中B细胞核呈棕黄色阳性，生发中心区域阳性细胞比例>40%；

阳性对照：扁桃体组织（生发中心B细胞强阳性，套区B细胞弱阳性）；

联合检测：与Bcl-6、CD10共染，OBF-1⁺Bcl-6⁺CD10⁺提示生发中心起源淋巴瘤。

WB B细胞系验证：

样本处理：Raji细胞（B淋巴瘤细胞系）用含1mM PMSF的RIPA裂解液冰上裂解30分钟，12000g离心15分钟取上清；

抗体孵育：单抗1:1000稀释（5% BSA+TBST），4℃孵育过夜，HRP二抗（1:5000）室温孵育1小时，28kDa处显清晰条带（Jurkat T细胞系为阴性对照）。

IF核定位验证：

与CD20（B细胞膜标志物） 共染，OBF-1（绿色，核定位）与CD20（红色，膜定位）在B细胞中共定位，DAPI复染细胞核（蓝色）。

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