商品属性

产品介绍This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]

商品详情

英文名称BRAF Rabbit pAb

中文名称B Raf抗体

别    名BRAF_HUMAN; Serine/threonine-protein kinase B-raf; EC:2.7.11.1; BRAF1; RAFB1; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1; B-Raf proto-oncogene, serine/threonine kinase; NS7; B-raf; B-RAF1; BRAF-1; 94 kDa B raf protein;  

研究领域肿瘤  信号转导  细胞凋亡  激酶和磷酸酶  

抗体来源Rabbit

克隆类型Polyclonal

克 隆 号

交叉反应Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Zebrafish,Chicken,Dog,Horse)

产品应用IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量84 kDa

检测分子量

细胞定位细胞核 细胞浆 细胞膜

性    状Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human BRAF: 21-120/766

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

应用范围：

免疫印迹（WB）、免疫组化（IHC-P/IHC-F）、免疫细胞化学（ICC）、ELISA等。

产品特性

特异性：

针对兜甲蛋白（Filaggrin或Loricrin）设计，经亲和层析纯化，可特异性识别目标抗原。

推荐稀释比例：

WB：1:50-400

IHC（石蜡切片）：1:10-100

ELISA：1:100-200

保存条件：

建议分装后于-20℃保存，避免反复冻融。

注意事项

用途限制：

仅限体外研究（In Vitro Research）使用，不可用于临床诊断。

质量控制：

部分产品通过免疫组化或Western Blot验证效价，建议预实验确定**条件。

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