商品属性

产品介绍This gene encodes a protein which is a member of the bromodomain-containing protein family. The product of this gene has been identified as a component of one form of the SWI/SNF chromatin remodeling complex, and as a protein which interacts with p53 and is required for p53-dependent oncogene-induced senescence which prevents tumor growth. Pseudogenes have been described on chromosomes 2, 3, 6, 13 and 14. Alternative splicing results in multiple transcript variants.

商品详情

英文名称BRD7 Rabbit pAb

中文名称鼻咽癌相关转录调节蛋白抗体

别    名75 kDa bromodomain protein; BP75; BRD 7; BRD7; BRD7_HUMAN; Bromodomain containing 7; bromodomain containing protein 7; Bromodomain-containing protein 7; CELTIX 1; CELTIX1; NAG4; Protein CELTIX-1.  

研究领域肿瘤  细胞生物  信号转导  细胞周期蛋白  表观遗传学  

抗体来源Rabbit

克隆类型Polyclonal

克 隆 号

交叉反应Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Dog)

产品应用IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量74 kDa

检测分子量

细胞定位细胞核

性    状Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human BRD7: 401-500/651

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

应用范围

免疫组化（IHC-P/IHC-F）：稀释比例1:400-800

免疫荧光（IF）：1:100-500

蛋白质印迹（WB）：1:500-2000

ELISA：1:500-1000

注意事项

用途限制：仅限科研使用，不可用于人类诊断或治疗。

实验建议：

建议预实验优化稀释比例，不同实验条件（如组织类型、固定方法）可能影响结果。

内参推荐使用巢式PCR内标引物（如SEQ ID NO.37/38）以排除假阴性。

相关检测技术背景

荧光探针设计：检测试剂盒中探针通常采用5′端荧光基团（如FAM、ROX）和3′端MGB淬灭基团，以提高信噪比。

反应体系：含Tris-HCl缓冲液、Mg²⁺、HotStart Taq酶等组分，适用于实时荧光PCR。

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