商品属性

产品介绍This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]

商品详情

英文名称Cadherin Recombinant Rabbit mAb

中文名称上皮钙粘附分子重组兔单抗

别    名Cadherin; CDH3; CDHP; Cadherin-1; Cadherin-2; Cadherin-3; Cadherin-4; Cadherin-5; CAM 120/80; CD144; CD324; CD325; Cdh4; CDH5; CDHE; CDHN; E-cadherin; N-cadherin; NCAD; Neural cadherin; P-cadherin; Uvomorulin; Vascular endothelial cadherin  

抗体来源Rabbit

克隆类型Recombinant

克 隆 号2B19

交叉反应Human,Mouse,Rat

产品应用WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量97 kDa

检测分子量

细胞定位细胞膜

性    状Liquid

浓    度1mg/ml

免 疫 原A synthesized peptide derived from human Cadherin: 850-906

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

靶点与功能

E-cadherin（CDH1）是钙依赖性跨膜粘附蛋白（分子量约120kDa），核心功能与研究价值包括：

上皮结构维持：通过胞外域同型粘附、胞内域与β-catenin/α-catenin结合，将细胞锚定到细胞骨架，维持上皮组织极性和完整性；

肿瘤转移抑制：在乳腺癌、胃癌、结直肠癌中表达下调或缺失，导致细胞间连接瓦解，EMT进程激活（如Snail/ZEB转录因子介导的E-cadherin启动子甲基化）；

预后标志物：肺癌、胰腺癌中E-cadherin低表达患者的5年生存率降低40%（临床数据），是病理诊断中判断肿瘤侵袭性的重要指标。

实验操作关键要点

WB检测EMT模型：

样本处理：TGF-β1（5ng/mL）诱导A549细胞EMT 48小时，RIPA裂解液（含1mM CaCl₂，维持E-cadherin构象）冰上裂解30分钟，12000g离心15分钟取上清；

电泳条件：7.5% SDS-PAGE凝胶（因分子量较大），恒压80V浓缩胶、120V分离胶，电泳90分钟；

抗体孵育：一抗1:1000稀释（5% BSA+TBST），4℃摇床孵育过夜，HRP二抗（山羊抗兔）1:5000室温孵育1小时，EMT组条带灰度值较对照组降低60%以上。

IHC-P病理诊断：

抗原修复：柠檬酸钠缓冲液（pH6.0）微波修复20分钟（石蜡切片），正常上皮组织细胞膜呈连续棕黄色阳性，肿瘤组织中可见“细胞膜信号缺失”或“胞质异位表达”；

阳性对照：结肠正常黏膜组织（腺上皮细胞膜强阳性）；

联合检测：与Vimentin（间质标志物）共染，E-cadherin⁻/Vimentin⁺双阳性提示EMT表型。

IF细胞定位观察：

与β-catenin抗体共染，E-cadherin（绿色）与β-catenin（红色）在细胞连接处共定位形成“线状信号”，EMT诱导后共定位系数从0.8降至0.2（共聚焦分析）。

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