商品属性

产品介绍Calreticulin is a highly conserved chaperone protein which resides primarily in the endoplasmic reticulum, and is involved in a variety of cellular processes, among them, cell adhesion. Additionally, it functions in protein folding quality control and calcium homeostasis. Calreticulin is also found in the nucleus, suggesting that it may have a role in transcription regulation. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin. Recurrent mutations in calreticulin have been linked to various neoplasms, including the myeloproliferative type.[provided by RefSeq, May 2020]

商品详情

英文名称Calreticulin Recombinant Rabbit mAb

中文名称钙网蛋白重组兔单抗

别    名Autoantigen RO; CALR; CALR protein; CALR_HUMAN; cC1qR; CRP55; CRTC; Endoplasmic reticulum resident protein 60; ERp60; grp60; HACBP; RO; Sicca syndrome antigen A; SSA.  

研究领域信号转导  细胞类型标志物  

抗体来源Rabbit

克隆类型Recombinant

克 隆 号3D1

交叉反应Human,Mouse,Rat

产品应用WB=1:2000-10000,IHC-P=1:200-800,IHC-F=1:200-800,IF=1:200-800,ICC/IF=1:100-200

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量44 kDa

检测分子量

细胞定位细胞浆 细胞膜 细胞外基质 分泌型蛋白

性    状Liquid

浓    度1mg/ml

免 疫 原A synthesized peptide derived from human Calreticulin: 45-86/417

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.02% Proclin300.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

应用范围及推荐稀释比例

Western Blot (WB)：1:500–2000

ELISA：1:500–1000

免疫组化（石蜡切片，IHC-P）：1:400–800

免疫组化（冰冻切片，IHC-F）：1:400–800

免疫细胞化学（ICC）：1:100–500

免疫荧光（IF）：1:100–500

保存条件

短期保存（1个月内）：2–8 ℃

长期保存（1年以上）：-20 ℃，避免反复冻融。

抗体特性

宿主：兔（Rabbit）

克隆类型：多克隆抗体（Polyclonal Antibody）

交叉反应：适用于人类（Human）样本，其他物种需验证。

产品用途

本产品仅供科研使用（For Research Use Only），不可用于临床诊断或治疗。

注意事项

使用前需离心处理，避免分装时浓度不均。

长期保存建议分装，避免反复冻融导致效价降低。

实验时需根据具体样本优化抗体稀释比例。

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