商品属性

产品介绍The caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). The processed form of Caspase 3 consists of large (17kDa) and small (12kDa) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 Ser29 and Asp175 Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (eg mantle zone B cells). Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.

商品详情

英文名称Caspase-3 Recombinant Rabbit mAb

中文名称半胱胺酸蛋白酶蛋白-3重组兔单抗

别    名cleaved Caspase 3; cleaved Caspase-3; APOPAIN; CASP3; Caspase 3 apoptosis related cysteine protease; Caspase3; CPP32; CPP32B; Cysteine protease CPP32; Human cysteine protease CPP32 isoform alpha mRNA complete cds; PARP cleavage protease; SCA 1; SCA1; SREBP cleavage activity 1; Yama; CASP3_HUMAN; Caspase-3; CASP-3; Apopain; Protein Yama; SREBP cleavage activity 1; SCA-1.  

抗体来源Rabbit

克隆类型Recombinant

克 隆 号4D2

交叉反应Human,Mouse,Rat

产品应用WB=1:1000-5000,IHC-P=1:200-800,IHC-F=1:200-800,IF=1:200-800

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量32 kDa

检测分子量12/29/32

细胞定位细胞浆

性    状Liquid

浓    度1mg/ml

免 疫 原A synthesized peptide derived from human Caspase 3: 176-277/277

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

靶点与功能

Caspase-3（半胱氨酸天冬氨酸蛋白酶-3）是凋亡通路的核心执行者（分子量：前体32kDa，活化后裂解为p17和p12亚基），核心功能与研究价值包括：

凋亡执行“剪刀”：被上游Caspase-8/9激活后，特异性切割PARP、ICAD等底物，导致DNA片段化、染色质凝聚、凋亡小体形成，是细胞凋亡的“最后执行者”；

疾病标志物：在肿瘤化疗敏感性（如顺铂诱导凋亡）、神经退行性疾病（如阿尔茨海默病神经元死亡）、心肌缺血再灌注损伤中，Caspase-3活化水平与病理程度正相关；

药物筛选工具：作为凋亡诱导药物（如紫杉醇、TRAIL）的药效评价指标，其活化率可量化药物诱导细胞死亡的效率。

实验操作关键要点

WB检测凋亡模型：

样本处理：Hela细胞经1μM STS（星形孢菌素）诱导凋亡6小时，RIPA裂解液（含1mM PMSF）冰上裂解30分钟，12000g离心15分钟取上清；

抗体稀释：一抗1:1000稀释（5% BSA+TBST），4℃孵育过夜，HRP二抗1:5000室温1小时，曝光显示17kDa（p17）和12kDa（p12）两条活化条带，未诱导组仅32kDa前体；

阳性对照：凋亡诱导的Jurkat细胞裂解液。

IHC-P组织凋亡定位：

抗原修复：柠檬酸钠缓冲液（pH6.0）微波修复20分钟（石蜡切片），肿瘤化疗后组织中凋亡细胞胞质呈棕黄色阳性（如胃癌化疗后癌巢边缘细胞）；

阴性对照：正常组织（如肺/肝实质细胞）或Caspase-3敲除小鼠组织；

联合检测：与TUNEL法共染，Caspase-3⁺/TUNEL⁺双阳性细胞占比>80%，验证凋亡特异性。

IF亚细胞定位：

与Cytochrome c（细胞色素c） 共染，凋亡早期Cytochrome c（红色）从线粒体释放至胞质，Caspase-3（绿色）随后活化并在胞质弥漫分布，共定位系数>0.6。

公司正在出售的产品