上皮钙粘附分子重组兔单克隆抗体

商品属性

产品介绍
英文名称E cadherin Recombinant Rabbit mAb

中文名称上皮钙粘附分子重组兔单克隆抗体

别    名E-cadherin; anion exchanger protein 3; Arc 1; Cadherin 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD 234; CD324; CD324 antigen; CDH1; CDHE; ECAD; Epithelial cadherin; epithelial calcium dependant adhesion protein; LCAM; Liver cell adhesion molecule; UVO; Uvomorulin; CADH1_HUMAN.  

抗体来源Rabbit

克隆类型Recombinant

克 隆 号B8F3

交叉反应Human

产品应用WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量97 kDa

检测分子量

细胞定位细胞膜

性    状Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human E-cadherin

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液PBS, Glycerol, BSA.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

产品介绍This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]

靶点与功能

E-cadherin（Epithelial Cadherin）是钙依赖性跨膜糖蛋白，分子量约120kDa，主要表达于上皮细胞，通过胞外域同型粘附、胞内域与β-catenin/α-catenin连接细胞骨架。其核心功能包括：

结构维持：稳定上皮组织结构极性，防止细胞离散；

肿瘤抑制：表达缺失是EMT的重要标志，与结直肠癌、乳腺癌等肿瘤的侵袭转移能力正相关；

信号转导：通过β-catenin调控Wnt通路，影响细胞增殖与凋亡。

实验操作关键要点

IHC-P优化（结直肠癌组织）：

抗原修复：采用柠檬酸钠缓冲液（pH6.0） 微波修复20分钟（95℃），增强膜/浆信号；

阳性定位：正常上皮细胞细胞膜/胞质呈连续棕黄色阳性，肿瘤组织中因EMT呈阴性或细胞膜信号断裂；

对照设置：必须包含正常结肠黏膜（强阳性）和间质纤维组织（阴性对照）。

WB检测注意事项：

样本处理：使用含1mM PMSF的RIPA裂解液冰上裂解30分钟，12000g离心取上清（避免蛋白酶降解）；

电泳条件：8% SDS-PAGE凝胶（因分子量较大），恒压80V浓缩胶、100V分离胶，目标条带约120kDa；

抗体孵育：一抗1:1000稀释（5% BSA+TBST），4℃摇床孵育过夜，HRP二抗（山羊抗兔）1:5000室温孵育1小时。

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