表皮生长因子受体抗体

商品属性

产品介绍
英文名称EGFR Rabbit pAb

中文名称表皮生长因子受体抗体

别    名Avian erythroblastic leukemia viral(v erb b) oncogene homolog; Avian erythroblastic leukemia viral(verbb) oncogene homolog; Cell growth inhibiting protein 40; Cell proliferation inducing protein 61; EGF R; Epidermal growth factor receptor(avian erythroblastic leukemia viral(v erb b) oncogene homolog); Epidermal growth factor receptor(erythroblastic leukemia viral(v erb b) oncogene homolog avian); Epidermal growth factor receptor; erbb 1; Erbb; Erbb1; HER1; mENA; Oncogene ERBB; PIG61; Receptor tyrosine protein kinase ErbB 1; Receptor tyrosine protein kinase ErbB1; Urogastrone; wa2; Wa5; EGFR_HUMAN.  

研究领域肿瘤  细胞生物  免疫学  生长因子和激素  

抗体来源Rabbit

克隆类型Polyclonal

克 隆 号

交叉反应Human,Mouse,Rat

产品应用WB=1:1000-5000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量175 kDa

检测分子量

细胞定位细胞核 细胞浆 细胞膜 分泌型蛋白

性    状Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human EGFR

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

产品介绍The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

抗体特性

宿主来源：兔多克隆抗体。

交叉反应：与人（Human）、小鼠（Mouse）、大鼠（Rat）、狗（Dog）等物种的AANAT蛋白具有反应性。

分子量：预测约23kDa（需通过Western Blot验证）。

免疫原：KLH偶联的人源AANAT蛋白片段（161-207aa）。

纯化方式：Protein A亲和纯化。

保存条件：

液体抗体：含0.01M PBS（pH 7.4）、10mg/mL BSA及0.1%叠氮化钠，建议-20℃保存，避免反复冻融。

冻干粉：室温下稳定至少1个月，-20℃可保存1年以上。

应用范围

推荐稀释比例（需用户优化）：

Western Blot（WB）：1:100-500

ELISA：1:500-1000

免疫组化（IHC-P/IHC-F）：1:100-500

免疫荧光（IF）：1:100-500

免疫沉淀（IP）：1:20-100

注意事项：

石蜡切片需进行抗原修复。

其他未标注的应用需用户自行验证。

研究领域

肿瘤学、细胞生物学、免疫学、神经生物学、信号转导及新陈代谢研究。

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