法尼基二磷酸合酶抗体

商品属性

产品介绍
英文名称FDPS Rabbit pAb

中文名称法尼基二磷酸合酶抗体

别    名FPPS_HUMAN; Farnesyl pyrophosphate synthase; FPP synthase; FPS; (2E, 6E)-farnesyl diphosphate synthase; Dimethylallyltranstransferase; Farnesyl diphosphate synthase; Geranyltranstransferase.  

研究领域肿瘤  细胞生物  信号转导  新陈代谢  

抗体来源Rabbit

克隆类型Polyclonal

克 隆 号

交叉反应Human,Mouse,Rat

产品应用WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量48 kDa

检测分子量

细胞定位细胞浆

性    状Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human FDPS: 201-300/419

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

产品介绍FDPS is a 419 amino acid enzyme belonging to the FPP/GGPP synthetase family. Localized to cytoplasm and peroxisome, FDPS expression is regulated by phorbol esters and polyunsaturated fatty acids. FDPS assists in cholesterol biosynthesis, post-translational protein modifications and synthesis of steroid hormones in the isoprenoid pathway.FDPS catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. FDPS is inactivated by interferon-induced RSAD2, which may result in the disruption of lipid rafts at the plasma membrane. Existing as a homodimer, FDPS may have anti-viral effects when inactivated by RSAD2. Reduced activity of FDPS in liver may partly be the cause of Zellweger syndrome and neonatal adrenoleukodystrophy, both of which are known to be peroxisomal deficiency diseases.

靶点与功能

FDPS是异戊二烯合成通路的关键酶，分子量约48kDa，催化异戊烯基焦磷酸（IPP）与香叶基焦磷酸（GPP）缩合生成法尼基焦磷酸（FPP），后者是胆固醇合成、蛋白法尼基化修饰的前体分子。其功能包括：

生理作用：参与细胞膜构建、信号通路激活（如Ras蛋白法尼基化）；

临床价值：肿瘤细胞中高表达，是双膦酸盐类药物（如唑来膦酸）的作用靶点，与骨转移癌治疗耐药性相关；

研究意义：通过抑制FDPS可阻断肿瘤细胞增殖，是抗肿瘤药物开发的重要分子标志物。

实验操作关键要点

WB验证（肝癌细胞裂解液）：

样本处理：使用含1mM PMSF的RIPA裂解液冰上裂解30分钟，12000g离心15分钟取上清；

电泳条件：10% SDS-PAGE凝胶，恒压120V分离胶，转膜选用0.45μm PVDF膜（250mA恒流1.5小时）；

抗体孵育：一抗1:800稀释（5%脱脂奶粉+TBST），4℃孵育过夜，HRP二抗（山羊抗兔）1:5000室温孵育1小时。

ELISA检测（血清样本）：

包被条件：抗体用碳酸盐缓冲液（pH9.6）稀释至2μg/mL，4℃包被12小时；

样本处理：血清1:100稀释，37℃孵育2小时，PBST洗涤5次；

显色控制：TMB底物显色15分钟，2M H₂SO₄终止，450nm读取OD值（标准曲线范围0.1-100ng/mL）。

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