商品属性

产品介绍This protein is a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Type I membrane protein. SIZE: 738 amino acids; 82536 Da. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Long isoform predominates all tissues examined, isoform Delta12 was detected only in trachea and isoform Delta14-15 only in lung, isoform Delta14 was detected in all tissues examined with the strongest expression in heart. PTM: Phosphorylated on Ser and Tyr residues after cellular activation. SIMILARITY: Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. CD31 is implicated in several functions, including transendothelial migration of leukocytes, angiogenesis, and integrin activation. Tyr-690 plays a critical role in leukocyte transendothelial migration (TEM) and is required for efficient trafficking of CD31 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. CD31 prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of CD31 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of CD31 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).

商品详情

英文名称CD31 Mouse mAb

中文名称血小板内皮细胞黏附分子1单克隆抗体

别    名platelet endothelial cell adhesion molecule precursor-1; PECAM-1; PECAM1; Adhesion molecule; CD31 antigen; CD31 EndoCAM; Endocam; FLJ34100; FLJ58394; GPIIA; Pecam 1; PECA1_HUMAN; PECAM 1 CD31 EndoCAM; PECA1; Pecam1; Platelet endothelial cell adhesion molecule; Platelet/endothelial cell adhesion molecule 1; Adhesion molecule; Platelet/endothelial cell adhesion molecule.

研究领域肿瘤  心血管  细胞生物  免疫学  干细胞  细胞粘附分子  血管内皮细胞  内皮细胞  

抗体来源Mouse

克隆类型Monoclonal

克 隆 号2D4

交叉反应Human

产品应用WB=1:1000-10000,IHC-P=1:200-2000,IHC-F=1:200-2000,IF=1:200-2000,Flow-Cyt=1ug/Test,ICC/IF=1:200-800

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量78 kDa

检测分子量

细胞定位细胞膜

性    状Liquid

浓    度1mg/ml

免 疫 原Recombinant human CD31 28-601/727aa (C-6x His-Tag) <Extracellular>

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

靶点与功能

CD31（PECAM1）是免疫球蛋白超家族成员（分子量约78kDa），核心功能与研究价值包括：

血管内皮细胞标志物：特异性表达于血管内皮细胞间连接（阳性率>95%），通过同源二聚体介导内皮细胞黏附，维持血管屏障完整性；在肿瘤新生血管中高表达，是评估肿瘤血管密度（MVD）的金标准（CD31⁺血管数与肿瘤转移风险正相关）；

免疫细胞功能：表达于血小板、单核细胞及部分T细胞，参与白细胞跨内皮迁移（炎症部位中性粒细胞通过CD31与内皮细胞结合并渗出血管）；

疾病关联：在动脉粥样硬化（斑块内新生血管CD31表达增加）、糖尿病视网膜病变（视网膜微血管CD31⁺细胞异常增殖）、瘢痕疙瘩（血管密度与复发率相关，临床研究显示CD31⁺血管数>20个/HPF患者复发风险升高2.8倍）中具有明确诊断价值。

实验操作关键要点

IHC-P肿瘤新生血管计数：

样本处理：乳腺癌石蜡切片，EDTA缓冲液（pH8.0）高压修复20分钟（优于柠檬酸盐缓冲液，保留胞间连接表位）；

抗体稀释：按1:200稀释（5% BSA+TBST），4℃湿盒孵育过夜，HRP二抗1:500室温孵育30分钟；

结果判读：CD31⁺血管以管腔结构或单个内皮细胞簇计数（棕色细胞膜着色），随机选取5个高倍视野（400×）计算MVD，平均值>15个/HPF提示高转移风险。

流式细胞术内皮细胞分选：

抗体标记：人脐静脉内皮细胞（HUVEC）用FITC标记CD31抗体（Biomart产品，1:50稀释）4℃避光孵育30分钟，流式分选CD31⁺内皮细胞（纯度可达95%以上）；

注意事项：避免使用含EDTA的缓冲液（可能导致CD31从细胞膜脱落），推荐使用PBS+2% FBS洗涤细胞。

WB血管内皮细胞裂解液检测：

样本处理：HUVEC细胞经10ng/mL VEGF刺激24小时（促进CD31表达），RIPA裂解液（含1mM PMSF）冰上裂解30分钟，12000g离心15分钟取上清；

抗体稀释：兔单抗按1:500稀释（5%脱脂奶粉+TBST），4℃孵育过夜，78kDa处显清晰条带（刺激组灰度值较对照组升高1.6倍）。

公司正在出售的产品