商品属性

产品介绍The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.

商品详情

英文名称CD34 Rabbit pAb

中文名称CD34抗体

别    名CD34 antigen; CD34 molecule; Cluster designation 34; Hematopoietic progenitor cell antigen CD34; HPCA1; CD34_HUMAN.  

研究领域肿瘤  心血管  免疫学  发育生物学  神经生物学  干细胞  细胞表面分子  糖蛋白  细胞类型标志物  血管内皮细胞  内皮细胞  

抗体来源Rabbit

克隆类型Polyclonal

克 隆 号

交叉反应Human,Mouse,Rat

产品应用WB=1:1000-5000,IHC-P=1:200-800,IHC-F=1:200-800,IF=1:200-800

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量39 kDa

检测分子量

细胞定位细胞膜

性    状Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human CD34: 301-385/385 <Extracellular>

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

靶点与功能

CD34是高度糖基化的I型跨膜黏蛋白（分子量105-120kDa），核心功能与研究价值包括：

造血干细胞标志物：特异性表达于造血干/祖细胞（HSC/HPC）、内皮祖细胞（EPC）表面，随细胞分化逐渐下调，是流式分选HSC的金标准（CD34⁺CD38⁻细胞群富含造血干细胞）；

血管生成标记：肿瘤新生血管内皮细胞高表达CD34，可通过IHC计数微血管密度（MVD）评估肿瘤恶性程度（MVD高的患者预后更差）；

细胞黏附作用：通过与选择素（L/E-选择素）结合，参与HSC归巢至骨髓微环境及炎症部位白细胞募集。

实验操作关键要点

流式细胞术（HSC分选）：

样本处理：人脐带血单个核细胞用PBS+2% FBS洗涤，Elabscience FITC-CD34抗体（E-AB-F1143C）1:50稀释，4℃避光孵育30分钟，流式分选CD34⁺CD38⁻细胞（占总细胞比例约0.1-0.5%）；

对照设置：同型对照（FITC-小鼠IgG1）确保非特异性结合率<1%，阳性对照选用健康人骨髓样本。

IHC-P肿瘤微血管密度（MVD）检测：

抗原修复：柠檬酸盐缓冲液（pH6.0）微波修复20分钟（石蜡切片），人肝癌组织中微血管内皮细胞细胞膜呈棕黄色阳性；

计数方法：×200视野下计数5个热点区域的CD34⁺血管数，取平均值即为MVD（>10个/HPF提示高侵袭性）；

抗体稀释：按1:100稀释（5% BSA+TBST），4℃孵育过夜，DAB显色5分钟。

WB造血干细胞蛋白检测：

样本处理：HUVEC细胞（人脐静脉内皮细胞，CD34⁺）用RIPA裂解液（含1mM PMSF）冰上裂解30分钟，MCE YA6360抗体1:1000稀释，4℃孵育过夜，110kDa处显清晰条带。

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