商品属性

产品介绍The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.

商品详情

英文名称CD34 Recombinant Rabbit mAb

中文名称CD34重组兔单抗

别    名CD34 antigen; CD34 molecule; Cluster designation 34; Hematopoietic progenitor cell antigen CD34; HPCA1; CD34_HUMAN.  

抗体来源Rabbit

克隆类型Recombinant

克 隆 号4G1

交叉反应Human,Mouse,Rat

产品应用WB=1:1000-5000,IHC-P=1:200-1000,IHC-F=1:200-1000,IF=1:200-1000,IP=1:20-100

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量39 kDa

检测分子量

细胞定位细胞膜

性    状Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human CD34: 350-385

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

靶点与功能

CD34（Cluster of Differentiation 34）是高度糖基化的I型跨膜蛋白（分子量约110kDa），核心功能与研究价值包括：

造血干细胞标志物：特异性表达于造血干/祖细胞（HSCs）、内皮祖细胞（EPCs）表面，是流式分选HSCs的金标准（CD34⁺细胞占骨髓单个核细胞比例约0.1-0.5%）；

细胞黏附与归巢：通过胞外域唾液酸残基结合L-选择素，介导HSCs与骨髓基质细胞黏附，调控干细胞归巢与定植；

血管生成指标：在肿瘤新生血管内皮细胞高表达，可通过IHC标记评估肿瘤微血管密度（MVD），预测抗血管生成药物（如贝伐珠单抗）疗效。

实验操作关键要点

流式分选造血干细胞：

样本处理：人脐带血单个核细胞用PBS+2% FBS洗涤2次，CD34抗体（FITC标记）1:50稀释，4℃避光孵育30分钟，流式分选CD34⁺CD38⁻Lin⁻干细胞群；

关键技巧：加入Fc受体封闭剂（如Human BD Fc Block™）减少非特异性结合，分选前过30μm滤网去除细胞团块。

IHC-P肿瘤微血管密度检测：

抗原修复：Tris/EDTA缓冲液（pH9.0）微波修复20分钟（石蜡切片），乳腺癌组织中肿瘤间质微血管内皮细胞细胞膜呈棕黄色阳性；

定量方法：随机选取5个高倍视野（400×），计数CD34⁺血管数目，取平均值即为MVD（微血管密度>10个/HPF提示预后不良）。

WB检测干细胞分化过程：

样本处理：CD34⁺细胞经粒细胞集落刺激因子（G-CSF）诱导分化0/7/14天，RIPA裂解液（含1mM PMSF+蛋白酶抑制剂）冰上裂解30分钟；

抗体稀释：按1:1000稀释（5% BSA+TBST），4℃孵育过夜，分化14天后CD34条带灰度值较未分化组降低80%（提示干细胞特性丢失）。

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