商品属性

产品介绍This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The genome is polymorphic at this locus with allele-specific splice variants encoding different isoforms, based on the presence/absence of long homologous repeats (LHRs). The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in this gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus, sarcoidosis and Alzheimer's disease. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. [provided by RefSeq, May 2020]

商品详情

英文名称CD35 Recombinant Rabbit mAb

中文名称Ⅰ型补体受体重组兔单抗

别    名C3 binding protein; C3b/C4b receptor; C3BR; C4BR; CD 35; CD35 antigen; complement component(3b/4b) receptor 1(Knops blood group); complement component(3b/4b) receptor 1 including Knops blood group system; Complement component receptor 1; Complement receptor 1; Complement receptor type 1; CR 1; CR1; CR1_HUMAN; KN; Knops blood group antigen.  

研究领域免疫学  细胞膜受体  淋巴细胞  t-淋巴细胞  b-淋巴细胞  

抗体来源Rabbit

克隆类型Recombinant

克 隆 号1A11

交叉反应Human

产品应用IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量274 kDa

检测分子量

细胞定位细胞膜

性    状Liquid

浓    度1mg/ml

免 疫 原A synthesized peptide derived from human CR1: 2000-2039

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

靶点与功能

CD35（Complement Receptor 1，CR1）是单次跨膜糖蛋白（分子量约190-280kDa，因剪切异构体而异），核心功能与研究价值包括：

补体清除受体：通过胞外域C3b/C4b结合位点，介导免疫复合物内化（主要在红细胞、巨噬细胞），是系统性红斑狼疮（SLE） 患者循环免疫复合物清除障碍的关键分子（CD35基因变异导致清除效率降低40%）；

B细胞共刺激：在滤泡树突状细胞（FDC）表面富集抗原-补体复合物，为B细胞提供**信号，促进生发中心形成和高亲和力抗体产生；

疾病关联：疟疾感染时，疟原虫通过CD35黏附红细胞导致 cytoadherence；HIV患者CD35⁺B细胞比例降低与抗体应答缺陷相关。

实验操作关键要点

流式细胞术（红细胞/白细胞分型）：

样本处理：人外周血红细胞用PBS+0.5% BSA洗涤（避免渗透压损伤），CD35抗体1:100稀释，4℃避光孵育30分钟，流式检测红细胞CD35表达强度（SLE患者较健康人降低35%）；

白细胞染色：PBMC中B细胞（CD19⁺CD35⁺）比例约5-10%，FDC（CD21⁺CD35⁺）可通过脾脏组织消化分离。

IHC-P免疫复合物定位：

抗原修复：柠檬酸盐缓冲液（pH6.0）微波修复20分钟（石蜡切片），人扁桃体组织中滤泡树突状细胞网络呈棕黄色阳性，B细胞区散在阳性；

封闭处理：5%驴血清+0.3% Triton X-100室温封闭1小时，一抗1:200稀释（5% BSA+TBST），4℃孵育过夜。

WB红细胞膜蛋白检测：

样本制备：健康人红细胞用低渗裂解液（5mM磷酸钠缓冲液pH7.4） 提取膜蛋白，12000g离心15分钟取沉淀，SDS-PAGE上样量需达80μg（因CD35在红细胞膜上丰度低）；

抗体稀释：重组兔单抗1:1000稀释，4℃孵育过夜，HRP二抗1:5000室温1小时，220kDa处显清晰条带（与Band 3蛋白共迁移，需通过IP验证特异性）。

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