GFAP单克隆抗体（工作液）

商品属性

产品介绍
英文名称GFAP(ready to use) Mouse mAb

中文名称GFAP单克隆抗体（工作液）

别    名Astrocyte; FLJ45472; GFAP; Glial Fibrillary Acidic Protein; Intermediate filament protein; GFAP_HUMAN.  胶质纤维酸性蛋白单克隆抗体

研究领域肿瘤  细胞生物  神经生物学  

抗体来源Mouse

克隆类型Monoclonal

克 隆 号7D8

交叉反应Human

产品应用WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量49 kDa

检测分子量

细胞定位细胞浆

性    状Liquid

免 疫 原Recombinant human GFAP full length,

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液0.01M PBS (pH7.4) with 1% BSA and 0.02% Proclin300.

保存条件Store at 2-8℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

产品介绍Glial fibrillary acidic protein (GFAP) is an intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS) including astrocytes and ependymal cells during development. GFAP has also been found to be expressed in glomeruli and peritubular fibroblasts taken from rat kidneys Leydig cells of the testis in both hamsters and humans, human keratinocytes, human osteocytes and chondrocytes and stellate cells of the pancreas and liver in rats. It is closely related to its non-epithelial family members, vimentin, desmin, and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton. GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells but its exact function remains poorly understood, despite the number of studies using it as a cell marker.

靶点与功能

GFAP（胶质纤维酸性蛋白）是III型中间丝蛋白，分子量约50-52kDa，主要表达于中枢神经系统星形胶质细胞。其核心价值包括：

细胞标记：作为星形胶质细胞的金标准标志物，用于区分胶质细胞与神经元（如与NeuN共染）；

病理意义：脑损伤、胶质瘤、多发性硬化等疾病中，GFAP表达上调并伴随胶质细胞活化（反应性星形胶质细胞增生）；

实验优势：工作液形式无需手动稀释，直接用于IHC/IF等实验，减少操作误差。

实验操作关键要点

IHC-P组织染色（脑切片）：

抗原修复：采用柠檬酸钠缓冲液（pH6.0） 微波修复20分钟（95℃），冷却至室温后PBS洗3次；

孵育条件：工作液滴加后37℃孵育30分钟（湿盒内避免干燥），无需4℃过夜；

对照设置：阳性对照用正常脑皮层组织（星形胶质细胞胞质阳性），阴性对照用肝组织（无表达）。

免疫荧光（IF）细胞标记：

细胞爬片固定：4%多聚甲醛固定15分钟，0.3% Triton X-100透化10分钟；

荧光二抗选择：搭配Alexa Fluor 488标记山羊抗小鼠IgG（1:1000稀释），DAPI复染细胞核；

结果观察：共聚焦显微镜下可见星形胶质细胞胞质呈绿色丝状网络结构。

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