商品属性

产品介绍The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]

商品详情

英文名称CD63 Recombinant Rabbit mAb

中文名称黑色素瘤相关抗原重组兔单克隆抗体

别    名Lysosomal associated membrane protein 3; CD63 antigen; CD63 antigen melanoma 1 antigen; CD63 molecule; granulophysin; LAMP 3; LAMP3; lysosome associated membrane glycoprotein 3; CD 63; CD63 antigen(melanoma 1 antigen); CD63_HUMAN; gp55; granulophysin; LAMP-3; LIMP; Lysosomal-associated membrane protein 3; Melanoma-associated antigen ME491; NGA; Ocular melanoma-associated antigen; PTLGP40; Tetraspanin-30; Tspan-30; Mast cell antigen AD1; ME491; melanoma 1 antigen; Melanoma associated antigen ME491; Melanoma associated antigen MLA1; MGC72893; MLA 1; MLA1; ocular melanoma associated antigen; OMA81H; Tetraspanin 30; Tspan 30; TSPAN30; Tumor biomarkers; Platelets markers.  

抗体来源Rabbit

克隆类型Recombinant

克 隆 号6C14

交叉反应Mouse

产品应用WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量26 kDa

检测分子量

细胞定位细胞浆 细胞膜

性    状Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from mouse CD63

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液PBS, Glycerol, BSA.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

关键靶抗原与抗体特性

黑色素瘤相关抗原（MAAs）重组兔单抗通过基因工程制备，针对黑色素细胞特异性标志物，核心靶点及抗体优势如下：

靶抗原 功能与表达特征 重组兔单抗优势

Melan-A/MART-1 黑色素细胞分化抗原，90%黑色素瘤（包括转移性）表达，正常组织仅见于黑素细胞 兔源重组抗体交叉反应低，IHC胞质染色清晰

gp100（HMB-45） 黑色素体跨膜糖蛋白，恶性黑色素瘤特异性标志物，与肿瘤侵袭性正相关 重组兔单抗亲和力高于鼠源，WB条带单一（100kDa）

PRAME 肿瘤睾丸抗原，黑色素瘤中过表达，与免疫治疗耐药相关 兔免疫系统可识别独特表位，IF定位更精准

SOX10 神经嵴发育转录因子，黑色素瘤/神经鞘瘤特异性表达，辅助鉴别诊断 重组抗体批次差异＜5%，IHC核定位信号强

实验应用关键操作

IHC诊断（转移性黑色素瘤组织切片）：

抗原修复：柠檬酸钠缓冲液（pH6.0）高温高压修复2分钟，自然冷却后3% H₂O₂阻断内源性过氧化物酶；

抗体孵育：Melan-A重组兔单抗1:200稀释（5% BSA+TBST），4℃孵育过夜，HRP二抗（山羊抗兔）1:5000室温孵育30分钟；

结果判读：黑色素瘤细胞胞质呈棕黄色颗粒状阳性，与S-100（胞核+胞质）联合染色可提高诊断准确率（S-100敏感性高但特异性低）。

WB验证（A375黑色素瘤细胞系）：

样本制备：RIPA裂解液（含1mM PMSF）冰上裂解30分钟，12000g离心取上清，上样量30μg总蛋白；

关键技巧：使用12% SDS-PAGE凝胶（分离小分子量蛋白），转膜后5%脱脂奶粉封闭1小时，一抗1:1000稀释4℃孵育过夜，曝光15秒可见20kDa条带。

FCM循环肿瘤细胞检测：

染色方案：PE标记Melan-A重组兔单抗与APC-CD45抗体（1:100）避光孵育20分钟（4℃），红细胞裂解后上机；

设门策略：CD45⁻Melan-A⁺为黑色素瘤循环肿瘤细胞（CTCs），正常外周血中应无阳性信号。

重组兔单抗技术优势

高特异性：兔免疫系统可识别更多构象表位，如PRAME的隐秘抗原表位，交叉反应率比鼠源抗体降低60%；

批次一致：重组表达避免杂交瘤细胞基因丢失问题，不同批次抗体WB灰度值变异系数＜8%；

多平台兼容：同一抗体可用于IHC/IF/WB，无需更换克隆号，减少实验变量。

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