组织相容性蛋白1单克隆抗体

商品属性

产品介绍
英文名称HLA-C Mouse mAb

中文名称组织相容性蛋白1单克隆抗体

别    名HLA-C; Major Histocompatibility Complex, Class I; HLA Class I Histocompatibility Antigen, C alpha Chain; HLA-JY3; D6S204; PSORS1; HLAC; Major Histocompatibility Antigen HLA-C; MHC Class I Antigen Heavy Chain HLA-C; Human Leukocyte Antigen-C alpha Chain; Psoriasis Susceptibility 1; Human Leukocyte Antigen C; HLA-C Antigen; HLA-Cw; HLC-C; MHC; HLAC_HUMAN.  

研究领域细胞生物  

抗体来源Mouse

克隆类型Monoclonal

克 隆 号3C1

交叉反应Human

产品应用WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500,Flow-Cyt=1ug/Test

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量4 kDa

检测分子量

细胞定位细胞膜

性    状Liquid

浓    度1mg/ml

免 疫 原Recombinant human HLA-C protein: 25-305/366

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

产品介绍HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. About 6000 HLA-C alleles have been described. The HLA system plays an important role in the occurrence and outcome of infectious diseases, including those caused by the malaria parasite, the human immunodeficiency virus (HIV), and the severe acute respiratory syndrome coronavirus (SARS-CoV). The structural spike and the nucleocapsid proteins of the novel coronavirus SARS-CoV-2, which causes coronavirus disease 2019 (COVID-19), are reported to contain multiple Class I epitopes with predicted HLA restrictions. Individual HLA genetic variation may help explain different immune responses to a virus across a population.[provided by RefSeq, Aug 2020]

靶点与功能

HLA-C是MHC I类分子的重要成员，由α链（45kDa）和β₂微球蛋白（12kDa）组成异二聚体，广泛表达于所有有核细胞表面。其核心功能与研究价值包括：

抗原呈递：结合内源性肽段（如病毒蛋白、肿瘤抗原），激活CD8⁺ T细胞介导的细胞毒性免疫应答；

移植免疫：HLA-C等位基因差异是移植物抗宿主病（GVHD） 的关键风险因素，抗体可用于供受者配型评估；

疾病关联：在HIV感染中，HLA-C通过限制病毒复制影响疾病进展；在结直肠癌中高表达与免疫逃逸相关。

实验操作关键要点

流式细胞术检测（外周血单个核细胞）：

样本处理：EDTA抗凝全血经Ficoll密度梯度离心分离PBMC，用含2% FBS的PBS重悬至1×10⁶细胞/mL；

抗体孵育：小鼠单抗1:50稀释，4℃避光孵育30分钟，2mL PBS洗涤2次（300g离心5分钟）；

阳性判定：CD8⁺ T细胞表面HLA-C表达率通常为70%-90%，可与CD3/CD8抗体进行三色流式分析。

IHC-P优化（肿瘤组织切片）：

抗原修复：采用EDTA缓冲液（pH9.0） 高压修复3分钟，增强细胞膜信号；

阳性定位：肿瘤细胞胞膜呈棕黄色线性阳性，正常组织中淋巴细胞、上皮细胞也可低表达；

对照设置：必须包含HLA-C敲除细胞系（阴性对照）和扁桃体组织（阳性对照，淋巴细胞高表达）。

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