HLA-DR重组兔单抗

商品属性

产品介绍
英文名称HLA-DR Recombinant Rabbit mAb

中文名称HLA-DR重组兔单抗

别    名DR alpha chain precursor; DRB1; DRB4; HLA class II histocompatibility antigen; HLA class II histocompatibility antigen DR alpha chain; HLA DR1B; HLA DR3B; HLA DRA; HLA DRA1; HLA DRB3; HLA DRB4; HLA DRB5; HLADR4B; HLADRA1; HLADRB; Major histocompatibility complex class II DR alpha; Major histocompatibility complex class II DR beta 1; Major histocompatibility complex class II DR beta 3; Major histocompatibility complex class II DR beta 4; Major histocompatibility complex class II DR beta 5; MGC117330; MHC cell surface glycoprotein; MHC class II antigen DRA; MHC II; DRA_HUMAN.

研究领域细胞生物  免疫学  

抗体来源Rabbit

克隆类型Recombinant

克 隆 号32G3

交叉反应Human

产品应用WB=1:1000-5000,IHC-P=1:1000-5000,IHC-F=1:1000-5000,IF=1:500-2000

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量29 kDa

检测分子量

细胞定位细胞浆 细胞膜

性    状Liquid

浓    度1mg/ml

免 疫 原A synthesized peptide derived from human HLA DRA: 150-190/254

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

产品介绍HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. [provided by RefSeq]

靶点与功能

HLA-DR是MHC II类跨膜糖蛋白，由α链（36kDa）和β链（27kDa）通过非共价键组成异二聚体，主要表达于专职抗原呈递细胞（APCs）表面，核心功能包括：

抗原呈递：结合外源性抗原肽（8-13个氨基酸），呈递给CD4⁺ T细胞，启动T细胞活化和免疫应答；

免疫调节：在自身免疫病（如关节炎）中，HLA-DR异常表达与自身抗原呈递相关；

疾病标志物：脓毒血症患者单核细胞表面HLA-DR表达下调，可作为免疫抑制状态的评估指标。

实验操作关键要点

流式细胞术检测（PBMC样本）：

样本处理：全血经Ficoll密度梯度离心分离PBMC，用含2% FBS的PBS重悬（浓度1×10⁶ cells/mL）；

抗体孵育：PE标记抗体1:100稀释，4℃避光孵育30分钟，洗涤后上流式细胞仪检测；

对照设置：同型对照（兔IgG-PE）、阳性对照（健康人PBMC中CD14⁺单核细胞HLA-DR高表达）。

IHC-P组织染色（淋巴结切片）：

抗原修复：采用EDTA缓冲液（pH8.0） 微波修复20分钟（95℃），冷却至室温后PBS洗3次；

封闭处理：5%驴血清+0.3% Triton X-100室温封闭45分钟，减少非特异性结合；

显色控制：DAB显色5-10分钟，阳性信号为棕黄色颗粒（主要位于APC细胞膜）。

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