商品属性

产品介绍Placental lactogens, also referred to as chorionic somatomammotropin hormones, are protein hormones. They are produced in the mammalian placenta and are similar in structure and function to growth hormones. Together, placental lactogens and growth factors play an essential role to assure successful lactation after pregnancy. Placental lactogens also modify the metabolic state of the mother during pregnancy to supply energy to the fetus. Placental lactogen I is a member of the somatotropin/prolactin family of hormones. The proteins in this family are crucial in mammalian growth control. Placental lactogen I is expressed primarily during mid-pregnancy, and it has been reported that DNA methylation regulates its tissue expression in rats. Placental lactogen II is expressed later in pregnancy and, in mice, its secretion is regulated by the inhibitory control of GH, the concentration of which increases rapidly at the beginning of the last half of pregnancy.

商品详情

英文名称Placental Lactogen Recombinant Rabbit mAb

中文名称人胎盘泌乳素重组兔单克隆抗体

别    名choriomammotropin; Chorionic somatomammotropin A; chorionic somatomammotropin; Chorionic somatomammotropin hormone 1(placental lactogen); chorionic somatomammotropin hormone 1; Chorionic somatomammotropin hormone 2; CS 1; CS-2; CS-B; CSA; CSB; CSH1; CSMT; hCS A; hCSB; Lactogen precursor; Lactogen, placental; PL; CSH1_HUMAN; placental lactogen.  

抗体来源Rabbit

克隆类型Recombinant

克 隆 号C8D6

交叉反应Human

产品应用WB=1:500-1000,IHC-P=1:200-1000,IHC-F=1:200-1000,IF=1:200-1000

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量22 kDa

检测分子量

细胞定位分泌型蛋白

性    状Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human Placental lactogen

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液PBS, Glycerol, BSA.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

靶点与功能

PLK1（Polo-like Kinase 1）是Polo激酶家族成员，通过磷酸化底物调控细胞周期关键进程：

G2/M期转换：磷酸化Wee1和Cdc25C，激活Cyclin B-Cdk1复合物，推动细胞进入有丝分裂；

纺锤体组装：定位于中心体和动粒，调控微管动态组装（敲除PLK1导致多极纺锤体和染色体分离异常）；

胞质分裂：参与收缩环形成和中体稳定性维持，其异常表达与非小细胞肺癌（58%）、乳腺癌（45%）、结肠癌（37%） 的化疗耐药密切相关，是PLK1抑制剂（如Volasertib）疗效评估的标志物。

实验操作关键要点

WB细胞周期同步化检测：

样本处理：HeLa细胞用** thymidine（2mM）** 双阻断法同步至G1/S期，释放后不同时间点收集（0h/G1期、6h/S期、12h/G2期、16h/M期），M期PLK1表达量为G1期的4.2倍；

电泳条件：10% SDS-PAGE凝胶，恒压80V浓缩胶、120V分离胶，转膜300mA恒流2小时；

抗体孵育：一抗1:1000稀释（5% BSA+TBST），4℃孵育过夜，HRP二抗1:5000室温孵育1小时，曝光时间30秒。

IHC-P临床样本分析：

抗原修复：EDTA缓冲液（pH9.0）高压修复10分钟（石蜡切片），阳性定位为细胞核（M期）和中心体（全周期）；

对照设置：乳腺癌组织芯片（阳性对照，核阳性率>50%）和PLK1敲除细胞系（阴性对照）；

评分标准：按细胞核染色强度（0-3分）和阳性细胞比例（0-4分）计算H-score（0-12分），H-score>6分提示预后不良。

IP激酶活性检测：

抗体用量：2μg抗体/500μg M期细胞裂解液，4℃旋转孵育4小时；

激酶反应：免疫沉淀复合物与ATP（100μM）、组蛋白H1底物30℃孵育30分钟，WB检测磷酸化组蛋白H1（p-H1 Ser10）。

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