BF405标记的溶酶体相关膜蛋白1（CD107）抗体

商品属性

产品介绍
英文名称LAMP1 Rabbit pAb, BF405 conjugated

中文名称BF405标记的溶酶体相关膜蛋白1（CD107）抗体

别    名CD107 antigen like family member A; CD107 antigen-like family member A; CD107a; CD107a antigen; LAMP 1; LAMP-1; LAMP1_HUMAN; LAMPA; LGP120; lgpA; Lysosomal membrane glycoprotein 120KD; Lysosomal Associated Membrane Protein 1; Lysosome associated membrane glycoprotein 1; Lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1;  

抗体来源Rabbit

克隆类型Polyclonal

交叉反应Human

产品应用IF=1:100-500, Flow-Cyt=2ug/Test, ICC/IF=1:50-200

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

性    状Lyophilized or Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human LAMP1 Rabbit pAb, BF405 conjugated

亚    型IgG

纯化方法affinity purified by Protein A

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

产品介绍Lysosome associated membrane protein (LAMP1), also known as lgp120 or lgpA, is a type 1 integral membrane protein that is transported from trans Golgi networks to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboxyl terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1. This mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation.

靶点与荧光标记特性

CD107（LAMP1）是溶酶体膜的I型整合膜蛋白，分子量约42kDa，核心功能包括溶酶体结构维持、膜转运及免疫细胞活化标志物（如NK细胞脱颗粒时膜转位）。BF405是蓝色荧光染料，激发波长405nm，发射波长450nm左右，适用于荧光显微镜、共聚焦成像及流式细胞术。

实验操作关键要点

免疫荧光（IF）活细胞成像：

细胞准备：选择贴壁细胞（如HeLa、RAW264.7），铺片密度50%-70%，无血清培养基饥饿处理2小时（减少背景）；

抗体孵育：抗体用含1% BSA的PBS稀释至1:500，37℃孵育30分钟（避免固定导致的结构破坏）；

成像条件：使用405nm激光激发，荧光滤镜选择450/50nm，与GFP（488nm）、Cy3（555nm）等荧光通道可多重标记。

固定细胞ICC实验：

固定与透化：4%多聚甲醛室温固定15分钟，0.1% Triton X-100透化5分钟（保持溶酶体结构完整）；

封闭条件：5% BSA + PBS室温封闭30分钟，降低非特异性结合；

对照设置：需设置未标记抗体组（排除自发荧光）和溶酶体酶（如Cathepsin D）共染组（验证定位特异性）。

流式细胞术检测NK细胞活化：

样本处理：NK细胞与靶细胞共培养4小时，加入抗体（1:200稀释）4℃避光孵育30分钟；

结果分析：CD107a阳性细胞比例反映脱颗粒活性，可与CD56、CD16等表面 marker 联合检测。

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