商品属性

产品介绍This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq, Jul 2008]

商品详情

英文名称PTEN Recombinant Rabbit mAb

中文名称一种肿瘤抑制基因抗体

别    名Bannayan Zonana; BZS a; ITGA 2; MGC11227; MHAM; MMAC 1; MMAC1; Multiple hamartoma(Cowden syndrome); Mutated in Mutiple Advanced Cancers 1; Phosphatase and Tensin Homolog; Phosphatidylinositol 345 trisphosphate 3 phosphatase and dual specificity protein phosphatase PTEN; Phosphatidylinositol 345 trisphosphate 3 phosphatase; Platelet antigen BR; PTEN 1; PTEN1; Tensin homolog; TEP 1; TEP1; VLA 2 Receptor alpha Subunit; 10q23del; Bannayan Zonana syndrome; BZS; DEC; GLM2; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; Phosphatase and Tensin Homolog; Phosphatase and Tensin Homolog; Phosphatase and tensin like protein; Phosphatidylinositol 345 trisphosphate 3 phosphatase and dual specificity protein phosphatase PTEN; Phosphatidylinositol-3, 4, 5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; PTEN 1; PTEN_HUMAN.  

研究领域肿瘤  细胞凋亡  

抗体来源Rabbit

克隆类型Recombinant

克 隆 号9D2

交叉反应Human,Mouse,Rat

产品应用WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1:20-100,ICC/IF=1:50-200,ELISA=1:5000-10000

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量44 kDa

检测分子量

细胞定位细胞核 细胞浆

性    状Liquid

浓    度1mg/ml

免 疫 原A synthesized peptide derived from human PTEN: 350-403

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

靶点与功能

Rad51（DNA修复蛋白RAD51同源物1）是RecA家族重组酶，通过ATP依赖的方式介导DNA双链断裂（DSB）的同源重组修复（HR），在基因组稳定性维持、减数分裂重组及复制叉保护中发挥核心作用。其异常表达与乳腺癌、卵巢癌（BRCA1/2突变相关）、白血病等恶性肿瘤的化疗耐药密切相关，是评估HR通路活性和PARP抑制剂疗效的关键标志物。

实验操作关键要点

WB优化（肿瘤细胞样本）：

样本处理：使用含蛋白酶抑制剂（1mM PMSF） 和磷酸酶抑制剂（PhosSTOP） 的RIPA裂解液冰上裂解30分钟，12000g离心15分钟取上清；推荐使用BRCA2突变型MDA-MB-436细胞作为阳性对照（Rad51高表达）；

电泳条件：10% SDS-PAGE凝胶，恒压80V浓缩胶、120V分离胶，电泳90分钟确保37kDa条带分离；

抗体孵育：一抗推荐1:2000稀释（5% BSA+TBST），4℃摇床孵育过夜，HRP标记二抗（山羊抗兔）1:5000室温孵育1小时，曝光时间30-60秒。

IHC-P注意事项（乳腺癌组织切片）：

抗原修复：采用EDTA缓冲液（pH9.0） 高压修复15分钟（95℃），增强核蛋白信号；

阳性定位：正常组织中精母细胞、胎盘滋养层细胞细胞核呈棕黄色阳性，肿瘤组织中可见细胞核/胞质异常表达；

封闭处理：5%驴血清+0.3% Triton X-100室温封闭45分钟，减少非特异性结合。

IF双标实验（DNA修复共定位）：

推荐与γH2AX抗体共染（DSB标志物），使用Alexa Fluor 488（绿色，Rad51核内灶点）和594（红色，γH2AX），激光共聚焦观察DNA损伤后二者共定位形成的修复灶（24小时照射γ射线后信号最强）。

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