微小染色体维持缺陷蛋白7重组兔单克隆抗体

商品属性

产品介绍
英文名称MCM7 Recombinant Rabbit mAb

中文名称微小染色体维持缺陷蛋白7重组兔单克隆抗体

别    名CDABP0042; CDC 47; CDC47; CDC47 homolog; DNA replication licensing factor MCM7; Homolog of S. cerevisiae Cdc47; MCM 2; MCM 7; MCM2; Mcm7; MCM7 minichromosome maintenance deficient 7; MCM7_HUMAN; Minichromosome Maintainence 7; Minichromosome maintenance complex component 7; Minichromosome maintenance deficient 7; Minichromosome maintenance protein 7; P1.1 MCM3; P1.1-MCM3; P1CDC47; P85MCM; PNAS 146; PNAS146.  

抗体来源Rabbit

克隆类型Recombinant

克 隆 号20E7

交叉反应Human

产品应用WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量79 kDa

检测分子量

细胞定位细胞核

性    状Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human MCM7

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液PBS, Glycerol, BSA.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

产品介绍The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008].

靶点与功能

MCM7是微小染色体维持蛋白家族（MCM2-7）成员，分子量约82kDa，作为DNA解旋酶复合物的催化亚基，参与DNA复制起始许可（Pre-replication complex）的形成，确保基因组在细胞周期中仅复制一次。其核心功能包括：

细胞增殖标志物：仅在增殖活跃的细胞（如干细胞、肿瘤细胞）中表达，G0期细胞不表达，是比Ki-67更灵敏的增殖活性指标；

肿瘤关联：在肺癌、乳腺癌、结直肠癌等实体瘤中高表达，与肿瘤分级、侵袭转移及预后不良正相关；

研究价值：可用于鉴别肿瘤组织中的增殖细胞亚群，辅助评估化疗药物对DNA复制的抑制效果。

实验操作关键要点

IHC-P肿瘤增殖检测（石蜡切片）：

抗原修复：采用柠檬酸盐缓冲液（pH6.0） 微波修复20分钟（95℃），增强核信号；

阳性定位：肿瘤组织中增殖区细胞胞核呈棕黄色阳性，正常组织中仅见于基底层或生发中心；

对照设置：需包含结肠腺癌组织（高增殖阳性对照）和正常肝组织（低增殖阴性对照）。

WB验证（细胞裂解液）：

样本处理：HeLa细胞（对数生长期）用含1mM PMSF的RIPA裂解液冰上裂解30分钟，12000g离心取上清；

电泳条件：10% SDS-PAGE凝胶，恒压80V浓缩胶、120V分离胶，转膜使用0.45μm PVDF膜（250mA恒流1.5小时）；

抗体孵育：一抗1:1000稀释（5%脱脂奶粉+TBST），4℃孵育过夜，HRP二抗（山羊抗兔）1:5000室温孵育1小时，曝光30-60秒（目标条带约82kDa）。

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