商品属性

产品介绍S100 beta is a member of the S100 family of proteins containing 2 EF-hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.

商品详情

英文名称S100B Mouse mAb

中文名称S100B蛋白单克隆抗体

别    名NEF; Protein S100 B; Protein S100-B; S 100 calcium binding protein beta chain; S 100 protein beta chain; S 100 protein beta subunit; S-100 protein beta chain; S-100 protein subunit beta; S100; S100 calcium binding protein B; S100 calcium binding protein beta(neural); S100 calcium binding protein beta chain; S100 calcium-binding protein B; S100 protein beta chain; S100B_HUMAN; S100beta; S100 β; S100-β; S100β.  

研究领域肿瘤  细胞生物  神经生物学  细胞周期蛋白  细胞分化  

抗体来源Mouse

克隆类型Monoclonal

克 隆 号1C6

交叉反应Human,Mouse,Rat

产品应用WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量10 kDa

检测分子量

细胞定位细胞核 细胞浆

性    状Liquid

浓    度1mg/ml

免 疫 原Recombinant human S100B protein: 2-92/92aa (N-6x His-Tag)

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

靶点与功能

S100P是S100家族钙结合蛋白成员，通过EF手型结构域结合Ca²⁺/Zn²⁺后发生构象变化，调控细胞增殖、侵袭及铁死亡抵抗。其核心功能包括：

肿瘤进展：通过激活RAGE受体促进上皮-间质转化（EMT），在胰腺癌中可增强肿瘤细胞迁移能力达2.3倍（临床样本数据）；

铁死亡抑制：与ACC1蛋白结合并促进其溶酶体降解，减少多不饱和脂肪酸合成，降低脂质过氧化水平；

免疫调节：上调促炎因子IL-6/TNF-α表达，增强巨噬细胞吞噬活性，参与肿瘤微环境重塑。

实验操作关键要点

WB优化（胰腺癌细胞系）：

样本处理：使用含Zn²⁺螯合剂（1mM EDTA） 的RIPA裂解液冰上裂解20分钟，避免金属离子干扰抗原-抗体结合；

电泳条件：15% SDS-PAGE凝胶（因分子量小），恒压100V电泳60分钟，确保10kDa条带与杂蛋白分离；

抗体孵育：一抗推荐1:1000稀释（5%脱脂奶粉+TBST），4℃摇床孵育过夜，HRP二抗（山羊抗兔）1:5000室温孵育1小时，曝光时间控制在15-30秒。

IHC-P注意事项（膀胱癌组织）：

抗原修复：采用柠檬酸钠缓冲液（pH6.0） 微波修复15分钟（95℃），避免EDTA缓冲液导致钙结合位点失活；

阳性对照：推荐使用胰腺导管腺癌组织芯片（癌细胞胞质呈弥漫性棕黄色阳性，正常导管上皮弱阳性）；

封闭处理：5%驴血清+2mM CaCl₂室温封闭30分钟，维持抗体结合活性。

IF双标实验（铁死亡研究）：

推荐与ACSL4抗体共染（脂质过氧化标志物），使用Alexa Fluor 488（绿色，S100P胞质定位）和594（红色，ACSL4），激光共聚焦观察铁死亡诱导后二者表达负相关性。

公司正在出售的产品