商品属性

产品介绍This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein encoded serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations in this gene may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

商品详情

英文名称Synapsin 1 Mouse mAb

中文名称神经突触素1单克隆抗体

别    名Brain protein 4.1; SYN 1; SYN 1a; SYN 1b; SYN I; SYN1; SYN1a; SYN1b; Synapsin 1; Synapsin1; SynapsinI; Synapsin-1; SYNI; SYN1_HUMAN.  

抗体来源Mouse

克隆类型Monoclonal

克 隆 号1F4

交叉反应Human,Rat (predicted: Mouse)

产品应用IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量85 kDa

检测分子量

细胞定位细胞浆 细胞膜

性    状Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human Synapsin I

亚    型IgG1, Kappa

缓 冲 液PBS, Glycerol, BSA.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

靶点与功能

P38（Synaptophysin，SYP）是突触小泡膜整合蛋白，核心功能与研究价值包括：

突触功能基石：通过4次跨膜结构域参与突触小泡的形成、运输及神经递质释放，是突触发生、可塑性及神经发育的关键标志物（在神经元突触前膜高度富集，可通过荧光强度量化突触密度）；

肿瘤诊断标志物：在小细胞肺癌（阳性率>90%）、神经母细胞瘤、类癌等神经内分泌肿瘤中特异性高表达，病理切片中呈“颗粒状胞质阳性”，是临床鉴别诊断的重要依据；

神经退行性疾病关联：阿尔茨海默病患者脑内P38表达量降低30%，与突触丢失程度正相关，可作为疾病进展的监测指标。

实验操作关键要点

IHC-P神经内分泌肿瘤诊断：

抗原修复：柠檬酸盐缓冲液（pH6.0）微波修复20分钟（石蜡切片），肿瘤细胞胞质呈棕黄色颗粒状阳性（小细胞肺癌典型特征）；

阳性对照：正常脑组织（海马CA3区神经元突触前膜强阳性）；

注意事项：与嗜铬粒蛋白A（CgA） 联合染色可提高诊断特异性（双阳性率>95%）。

WB检测（突触体提取样本）：

样本处理：大鼠脑组织经蔗糖密度梯度离心法提取突触体，RIPA裂解液（含1mM PMSF）冰上裂解30分钟；

电泳条件：12% SDS-PAGE凝胶，恒压80V浓缩胶、120V分离胶，电泳90分钟；

结果特征：38kDa处显带，突触体组分中灰度值是胞质组分的5-8倍。

IF突触共定位实验：

与突触素I（Synapsin I） 共染，P38（绿色，突触小泡）与Synapsin I（红色，突触前膜骨架）在神经元轴突末端共定位，形成“串珠状荧光信号”（代表成熟突触）。

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